276 
Journal of Agricultural Research 
Vol. I, No. 4 
varying climatic conditions. In some particulars, however, the conclu¬ 
sions which seemed justifiable from their experiments are not borne out 
by these more extensive data. 
The experiments discussed in this article were designed to study fur¬ 
ther the environmental influences and to show the r 61 e exerted by the 
soil and the part played by climatic conditions, such as rainfall, sunshine, 
humidity of the atmosphere, temperature, winds, and elevation above 
sea level. As in the case of the previous experiments, 1 they were car¬ 
ried on in cooperation with the Office of Cereal Investigations of the 
Bureau of Plant Industry. The agricultural experiment stations of 
Maryland, Kansas, and California cooperated by growing the crops. 
CONDUCT OF THE EXPERIMENTS 
In order to distinguish between the role played by soil and that by 
environment other than soil, samples of soil were interchanged among 
three localities, Maryland (College Park), Kansas (Hays), and California 
(Davis), which differ widely in climatic conditions. From each locality 
sections of a normally fertile wheat-producing soil 5 feet square and 3 feet 
deep were dug up in 3-inch layers, sacked, and replaced in the same 
original position. To obviate any differences due to this manipulation 
a portion of soil 5 feet square and 3 feet deep from each locality was like¬ 
wise dug up in 3-inch layers, sacked, and stored until the soils from the 
two other localities had arrived, when all three samples were placed in 
their respective positions. A fourth plat of soil of the same size was 
allowed to remain undisturbed in each locality to determine whether the 
treatment to which the three other soils had been subjected would exert 
any influence on the composition of the grain. Thus, there were 12 
experimental plats, 4 in each locality, as shown in the following plan: 
California: 
Plat of 
Plat of 
Plat of 
Plat of 
Kansas: 
Plat of 
Plat of 
Plat of 
Plat of 
Maryland: 
Plat of 
Plat of 
Plat of 
Plat of 
TWELVE EXPERIMENTAL, PEATS 
undisturbed California soil, or check plat. 
disturbed California soil.], , , . . , , , , , . 
(Each taken up m 3-inch layers and replaced m 
I original order. 
Kansas soil. 
Maryland soil. 
undisturbed Kansas soil, or check plat. 
disturbed Kansas soil.] — , A , . . - . , , 
California soil [Each taken up m 3-inch layers and replaced m orig- 
Maryland soil. 
inal order. 
undisturbed Maryland soil, or check plat. 
disturbed Maryland soil.] — , , , . . , , ' , , , . 
California soil [Each taken up m 3-inch layers and replaced m 
T;r - ... [ original order. 
Kansas soil. J b ■* 
1 he Clerc and heavitt. Op. dt. 
