Mar. 25, 1914 
Imperfect Fungi from Wheat, Oats, and Barley 
477 
Table I. —Results of inoculating seedling leaves of wheat , barley , oats, and rye with 
imperfect fungi obtained from cereals. 
Test 
No. 
Species. 
Origin. 
Inocu¬ 
lated 
on— 
Date of 
inocula¬ 
tion. 
Length 
of 
incu¬ 
bation. 
Num¬ 
ber of 
inocu¬ 
la¬ 
tions. 
Infection. 
Control. 
Num¬ 
ber. 
Per 
cent- 
age. 
Total 
num¬ 
ber. 
Num¬ 
ber in¬ 
fected. 
1911. 
Days. 
1 
Helminthospo- 
Wheat node 1 , 
Wheat.. 
Oct. 31 
6 
21 
21 
100 
11 
i 
rium grami- 
neum. 
2 
.do. 
.do. 
...do.... 
Nov. 10 
4 
33 
17 
Si 
12 
0 
3 
.do.. 
.do. 
Barley.. 
Oct. 31 
6 
23 
84 
.do. 
.. ,do. 
Nov. 10 
78 
5 
.do. 
.do. 
Oats.... 
Oct. 31 
6 
l6 
66 
6 
.do. 
.do. 
.. .do. 
Nov. 10 
6 
7 
.do. 
.do. 
Rye. 
.. .do..... 
36 
8 
14 
1912. 
8 
.do. 
Barley leaf l ... 
Wheat.. 
Jan. 24 
s 
43 
40 
93 
is 
0 
.do. 
.do. 
Barley.. 
.. .do. 
67 
67 
10 
.do. 
.do. 
Oats.... 
. ..do. 
5 
SO 
50 
100 
20 
0 
11 
.do. 
.do. 
Rye. 
.. .do. 
100 
0 
12 
Cladosporium 
Oat leaf 1 . 
Wheat.. 
.. .do. 
35 
gramineum. 
13 
.do. 
.do. 
...do. 
.. .do. 
6 
14 
.do. 
.do. 
Barley.. 
.. .do. 
. 
15 
.do. 
.do. 
...do. 
.. .do. 
6 
64 
16 
.do. 
.do. 
Oats.... 
...do. 
17 
.do. 
.do. 
.. .do. 
...do. 
6 
18 
.do. 
.do. 
Rye. 
...do. 
5 
37 
0 
19 
.do. 
.do. 
... do .... 
...do. 
6 
32 
20 
Fusarium cul- 
Oat seedling s . 
Wheat.. 
Mar. 19 
50 
morum. 
21 
.do. 
.do. 
Barley.. 
...do. 
14 
22 
.... .do. 
.do. 
Oats.... 
...do. 
*3 
.do. 
.do. 
Rye. 
.. .do. 
14 
So 
1 From University Farm, St. Paul, Minn. * From farm of Mr. Peter Hanson, Sandy, Utah. 
Table I shows that the strains of Helminthosporinm gramineum from 
both wheat and barley infected the leaves of wheat, barley, oats, and rye. 
On wheat, barley, and rye the leaf spots at the point of inoculation became 
distinct in a little less than three days after inoculation. These spots, 
which had a dead central area surrounded by a brown margin, slowly 
increased in size until their diameter was almost equal to the width of 
the leaf. A tendency to striation of the leaf area contiguous to the 
spots was noticed. No striking difference could be detected in the effect 
of the fungus from wheat or barley, the strain from wheat attacking 
barley and rye fully as severely as the strain from barley, and the strain 
from barley attacking wheat and rye fully as severely as the strain from 
wheat. On oats the two strains showed a slight difference in virulence, 
the fungus isolated from the barley apparently showing greater vigor in 
its attack than the fungus from wheat. In fact, three days after inocu¬ 
lation with the fungus from barley, oat leaves were so severely affected 
that in many cases they were cut in two, the tip portion often breaking 
off and falling to the ground. The two strains behaved so similarly, 
however, that physiologically they undoubtedly may be regarded as iden¬ 
tical. Morphologically, no difference was detected. 
