508 
Journal of Agricultural Research 
Vol. I, No. 6 
far as it is possible to make comparisons, the reactions given agree very 
closely with our nonliquefying udder cultures. 
Table VIII .—Results of fermentation tests of Streptococcus pyogenes described in the 
literature. 
A still further comparison is possible by the tabulation of the fermen¬ 
tation reactions of five typical cultures of Streptococcus pyogenes obtained 
through the courtesy of Prof. C. E. A. Winslow, of the American Museum 
of Natural History. These results are given in Table IX. Although 
some of these cultures have been grown on artificial media for many 
years, they still exhibit the same general characters as our freshly isolated 
udder cultures—namely, an ability to ferment dextrose, saccharose, and 
lactose, general failure to ferment raffinose and the polysaccharids, and 
an erratic tendency to ferment the alcohols. Unfortunately the gelatin 
test was not made on these five cultures. The fermentation of glycerin 
by three of the five indicates that they may have been of the liquefying 
type. Savage in 176 cultures of streptococci isolated from cases of mastitis 
found that 95 per cent liquefied gelatin. 1 His cultures differed from 
both the typical S. pyogenes and our liquefying cultures in that 49 per 
cent fermented raffinose. 
Table IX. —Results of fermentation tests of five cultures of Streptococcus pyogenes from 
American Museum of Natural History {New York ) collection . 
Source. 
Dextrose. 
1 
i 
i 
0 
Lactose. 
1 
Starch. 
Inulin. 
<U 
M 
1 
Glycerin. 
New York Post Graduate Medical College (fatal sep¬ 
ticemia). 
3*90 
3*8 5 
3* 60 
0.20 
0.18 
0.23 
0.35 
o- 59 
Dr. Bien, Chicago. Ill. (abscess in erysipelas). 
5*45 
4 * 
75 
3*25 
0 
• 13 
.18 
2 -55 
2.34 
Boston Board of Health (urine). 
3*85 
4 * 05 
•45 
0 
3* 98 
0 
0 
1-74 
Johns Hopkins University. 
6 .45 
4 - 95 
4 * 70 
. 20 
•45 
.08 
4 * 91 
I. 67 
Michigan Agricultural College. 
2. 50 
0 
1* IS 
•OS 
O 
.09 
•23 
.19 
VARIATION FROM TYPE IN THE UDDER ORGANISMS 
The trouble from infected udders at both the Beltsville and Annapolis 
farms was in the nature of an epidemic. The infection apparently 
spread from cow to cow and became so severe that at Annapolis one or 
1 Savage. W. G.. Report upon the bacteriology and pathology of “Garget*' (or mastitis) in cows. 37th 
Ann. Rept. Local Govt. Bd. [Gt. Brit.], 1907-8. Suppl. Rept. Med. Off., pp. 359-424* *909. 
