4 8 
Journal of Agricultural Research 
Vol. II, No. i 
large part of the cells, however, are practically free from any color, 
this being the cause of the very irregular streaked appearance of the 
seed coat. The cells are extremely irregular in shape, which gives to a 
section through the palisade layer a marked unevenness of appearance. 
The basal-color layer has a pale dull-yellow pigment massed in its 
lower cells. A good number of the cells seem to be colorless. The 
variety is the second generation of a cross of a Watson No. 5 on a 
Coffee No. 16. 
One other cowpea needs to be mentioned, No. 0618. It is pale buff or 
day, dusted over with brown gray on the Watson pattern. It is further 
pigmented with very small, round, deep, purple-brown dots, similar to 
the color massed around the 41 ‘eye.” The pigmentation of the palisade 
cells is confined to a little over half their number, the pigment being in 
the lower third of the cell cavity. It is a blue alkaline anthocyanin, with 
little or no trace of the acid form of this pigment, although in the minute 
areas represented by the deep purple-brown dots of the seed coat a small 
amount of red acid anthocyanin seems to be present. All the palisade 
cells are strongly contorted. The underlying basal layer is narrow and 
also strongly contorted. It is largely destitute of pigment, except for 
segregated masses of a deep-orange color located in widely separated 
groups of cells in the upper portion of the layer. The parentage of this 
hybrid is unknown. Tike No. 5, previously mentioned, it was obtained 
from Mr. J. W. Trinkle, of Madison, Ind., but correspondence has failed 
to give any data regarding its origin. 
III.-COWPEAS HAVING ONEY A MEEANIN-EIKE PIGMENT IN THE PAUSADE 
EAYER 
The third class of cowpeas—namely, those in which a melanin-like pig¬ 
ment alone is found in the palisade layer—ranges through various shades 
of light brown, buff, and red. 
The first of these is a pale-buff or clay-colored cowpea, No. 237-3-7. 
A minute quantity of melanin-like pigment was detected in the palisade 
cells, and that only in the case of darker specimens. The very pale buff- 
colored seeds show no trace of pigmentation in this layer. The effect of 
this minute trace of pigment on the general color scheme of the seed coat 
must be small. Indeed, the color is easily explained by the stronger 
pigmentation of the basal-color layer. This layer is a vivid brownish 
yellow color. All the tests for anthocyanin failed to show a trace of this 
prevailing pigment in any cells of this cowpea. 
It should be noted that the presence of melanin-like pigment in the 
palisade cells is of some interest in regard to the affinities of this cowpea 
to others in which it is also found, its parentage being Red No. 4 crossed 
on Taylor No. 14, second generation from the cross. 
Another practically monochrome cowpea is No. 27544, known as the 
Iron cowpea. It ranges from a delicate buff or clay to a strong reddish 
