Apr. 15, 1914 
Pycnospores of Chestnut-Blight Fungus 
69 
Table) I .—Longevity tests of pycnospores in soil in the field in 1913. 
[Samples taken from bases of diseased chestnut trees bearing pycnospore traps.] 
Series A. 
Trap No. and 
soil sample No. 
Rainfall 
Date. 
| Inches. 
Date of 
collection. 
Date of 
cultures. 
Period of dry¬ 
ing. 
Condition of soil 
when tested. 
Number of 
v i able 
spores per 
gram of 
soil. 
Trap I: 
Sample 1.. 
Sample 3. . 
jMar. 20 
I. 64 
fMar. 21 
[Mar. 24 
Mar. 21 
Mar. 24 
Days 
1 
4 
Wet from rain.. 
Nearly air-dry . 
I, 140, OOO 
47, 072 
Trap III: 
Sample 2.. 
Sample 4.. 
j. .do. 
I. 64 
fMar. 21 
\Mar. 24 
Mar. 21 
Mar. 24 
1 
4 
Wet from rain.. 
Nearly air-dry . 
5 2 7 > 7 2 4 
21, 509 
Series B. 
Trap I: 
Sample 5.. 
Sample 7.. 
Trap III: 
Sample 6.. 
Sample 8.. 
Apr. 
5 
% 
1 
Wet from rain.. 
3,604, OOO 
Apr. 
7 
Damp. 
7,128, OOO 
Apr. 
5 
K 
Wet from rain.. 
4,410, OOO 
Apr. 
7 
2% 
Damp. 
1,998, OOO 
Series C. 
Trap I: 
Sample 9.. 
|Apr. io~ 
4. 06 
fApr. 18 
[Apr. 21 
Apr. 19 
2 % 
Damp from rain 
6, 570,000 
Sample 11. 
Apr. 22 
sK 
Air-dry. 
I, 640, OOO 
Trap III: 
Sample 10. 
Sample 12. 
j. .do. 
4. 06 
fApr. 18 
\Apr. 21 
Apr. 19 
Apr, 22 
2'/ t 
sV* 
Damp from rain. 
Air-ary. 
2,412, 666 
288, OOO 
Series D. 
Trap I: 
Sample 
* 3 - 
lApr. 27- 
2.43 
Apr. 
28 
Apr. 
29 
0 
Very wet. 
1,209,500 
Sample 
I 7 • 
[ 28. 
■ May 
5 
May 
6 
6 
Air-dry. 
2, 723 ,333 
Sample 
21. 
jApr. 29 
. 11 
May 
12 
May 
13 
13 
Loose, air-dry 
surface soil 
exposed to 
sun. 
h 077 ,333 
Trap III: 
Sample 
14. 
]Apr. 27- 
2.43 
fApr. 
28 
Apr. 
29 
0 
Very wet. 
2,948, OOO 
Sample 
18. 
[ 28. 
<May 
3 
May 
6 
6 
Air-dry. 
86,000 
Sample 
22. 
JApr. 29 
. 11 
[May 
12 
May 
13 
13 
Loose, air-dry 
surface soil 
partly shaded. 
84, OOO 
Trap IV: 
Sample 
* 5 - 
Apr. 27- 
2.43 
fApr. 
30 
May 
1 
1 
Wet from rain.. 
3,336, OOO 
Sample 
19. 
28. 
<May 
5 
May 
6 
6 
Air-dry. 
3,792, OOO 
Sample 
2 3 - 
Apr. 29 
. 11 
[May 
12 
May 
13 
13 
Loose, air-dry 
surface soil 
exposed to 
sun. 
2,412,333 
