June is, 1914 
Flavor of Cheddar Cheese 
173 
groups have been maintained on agar slopes, with transfers made every 
month or six weeks. The constancy of the reactions of these cultures 
on the five organic substances has been tested, and the results on salicin, 
mannit, sucrose, and glycerin are given in Table II. The results on 
lactose are omitted, because they are always positive. 
Table II .—The constancy of the Bacterium lactis acidiand coccus groups in their action 
upon test substances 
Bacterium Lactis Acidi 
Production of acid in broth containing— 
No. of 
culture. 
Salicin. 
Mannit. 
Sucrose. 
Glycerin. 
First 
test. 
Age of 
culture 
at 
time of 
second 
test. 
Sec¬ 
ond 
test. 
Age of 
culture 
at 
time of 
third 
test. 
Third 
test. 
First 
test. 
Sec¬ 
ond 
test. 
Third 
test. 
First 
test. 
Sec¬ 
ond 
test. 
Third 
test. 
First 
test. 
Sec¬ 
ond 
test. 
Third 
test. 
1. 
Months. 
8 
Months. 
12 
_ 
3 
4- 
_ 
4- 
__ 
_ 
! _ 
_ 
_ 
9 
4- 
12 
+ 
4- 
-f 
_ 
_ 
_ 
_ 
_ 
4- 
1 
+ 
_ 
_ 
_ 
_ 
_ 
5. 
4- 
4 
4- 
8 
+ 
_ 
4- 
_ 
_ 
_ 
_ 
_ 
_ 
6. 
4 
4- 
8 
-f 
-f 
4- 
4* 
7. 
4- 
4 
4- 
8 
-f 
+ 
4- 
_ 
_ 
_ 
_ 
_ 
_ 
8. 
-f 
+ 
4- 
-f 
+ 
4- 
_ 
_ 
Streptococcus 
Production of acid in broth containing— 
No. of 
culture. 
Salicin. 
Sucrose. 
Glycerin. 
Mannit. 
First 
test. 
Age of 
culture 
at 
time of 
second 
test. 
Sec¬ 
ond 
test. 
1 
Age of 
culture 
at 
time of 
third 
test. 
1 
Third 
test. 
First 
test. 
Sec¬ 
ond 
test. 
Third 
test. 
First 
test. 
Sec¬ 
ond 
test. 
Third 
test. 
First 
test. 
Sec¬ 
ond 
test. 
Third 
test. 
Months. 
Months. 
9 . 
— 
10 
— 
14 
— 
— 
4 - 
4 - 
— 
—- 
— 
— 
— 
— 
10. 
— 
10 
_ 
14 
4- 
__ 
-f* 
_ 
_ 
11. 
_ 
6 
_ 
4 - 
4 - 
— 
_ 
_ 
_ 
, _ 
12. 
_ 
5 
_ 
4 - 
4 - 
4- 
4 - 
_ 
13. 
_ 
9 
— 
4 - 
4 - 
4 - 
4 - 
_ 
_ 
14. 
-f 
9 
4- 
12 
4 - 
_ 
_ 
_ 
_ 
_ 
*5 . 
4 - 
4 
4 - 
3 
4 - 
— 
— 
— 
4 - 
4 - 
4 - 
— 
_ 
— 
16. 
+ 
4 
4 - 
8 
4 - 
4 - 
4 - 
4 - 
— 
— 
— 
— 
— 
— 
17. 
4. 
5 
4 - 
4 - 
4 - 
_ , 
_ 
4- 
4- 
18. 
6 
9 
_ 
4- 
4- 
* 4 * 
4- 
4 * 
4- 
4- 
4- 
4- 
19. 
4 - 
5 
+ 
9 
4- 
4 - 
4- 
4- 
4. 
20 . 
4 - 
4 
4 - 
8 
4 - 
4 * 
4 - 
4- 
4 - 
4 - 
4 - 
4 - 
4 - 
4 - 
