Jan. a, 1917 
Diagnosis of Tuberculosis by Complement Fixation 
9 
Table II .—Titration for anticomplementary dose 
Tube 
No. 
Antigen. 
Inacti¬ 
vated 
normal 
serum. 
Comple¬ 
ment, s 
per cent. 
Physio¬ 
logical 
salt so¬ 
lution. 
Incu¬ 
bation 
period. 
Hemolysin. 
Erythro¬ 
cytes, 
3 -S per 
cent 
Incu¬ 
bation 
period. 
Degree of 
hemolysis. 
C. c. 
C.c. 
C. c. 
C . c. 
1 
o- 5 
0. 2 
1 
* 
1 c. c. con- 
1 
Incomplete. 
V 0 
taining 2 
g . 
units. 
2 
• 4 
. 2 
1 
.do. 
1 
Complete. 
3 
• 3 
. 2 
1 
& 
§ 
1 
g 
Da 
2 
*44* 
.do. 
1 
Do. 
' I 
la 
.... .do. 
1 
w 
Do. 
5 
6 
. 00s 
. 2 
1 
V 6 
jd 0 
.do. 
1 
Do. 
7 
None. 
. 2 
1 
d +J 
1 
Da 
8 
. 1 
None. 
m 
1 
Da 
Table III .—Titration for hemolytic properties 
Tube No. 
Antigen. 
Inacti¬ 
vated 
normal 
serum. 
Erythro¬ 
cytes. 
Physio¬ 
logical 
salt so¬ 
lution. 
Incuba¬ 
tion. ■ 
Degree of 
hemolysis. 
C. c. 
o- 6 
C. c. 
o- 2 
C. c. 
1 
.M *0 
Very faint. 
* 5 
. 2 
1 
s« 
None. 
. 4 
. 2 
1 
§44 
Do. 
. 3 
. 2 
1 
ui 
Do. 
. 2 
. 2 
1 
§ 
Do. 
6 . 
. 1 
. 2 
1 
g s 
A 
Do. 
* 05 
. 2 
1 
£ 6 
Do. 
8 ... 
None. 
Do. 
. 3 
None. 
<8 
METHOD 2: STANDARDIZATION OF BACILLARY EMULSION WITH A FIXED AMOUNT OF 
TUBERCULIN PRECIPITATE 
Solutions containing io, 20, 30, 40, etc., mgm., in 10 c.c. of a 0.6 per 
cent solution of sodium chlorid were prepared from the tuberculin pre¬ 
cipitate described under the preparation of antigen. Tests were under¬ 
taken with the various dilutions, 0.1 c. c. of each serving as a basic amount 
of the antigen to which increasing amounts of the bacillary emulsion had 
been added in the different tubes. Tables IV to VI demonstrate the 
procedure of the tests. 
Table IV ,—Titration for antigenic dose ■. 
Dose 
No. 
10 mgm. 
tubercu¬ 
lin pre¬ 
cipitate 
in ioc. c. 
sodium- 
cblorid 
solution.® 
Bacil¬ 
lary 
emul¬ 
sion. 
Inacti¬ 
vated 
positive 
serum. 
Com¬ 
ple¬ 
ment, 
5 Per 
cent. 
Physio¬ 
logical 
salt 
solution. 
Incu¬ 
bation. 
Hemo¬ 
lysin 
(1 unit= 
0.5 c. c.) 
Ery¬ 
thro¬ 
cytes, 
3-5 Per 
cent 
Incuba¬ 
tion. 
Degree of 
hemolysis. 
1 . 
C. c. 
& 0.1 
C. c. 
0.40 
C.c. 
0.20 
C. c. 
1 
>> 
V 
2 units.. 
C. c. 
1 
None. 
2. 
6.1 
•30 
. 20 
. 20 
1 
.. .do. 
1 
d 
Do. 
& .1 
. 20 
1 
cf « 
u 
...do. 
1 
§ 
Do. 
. . 
A _ . 
. 1 
•15 
. 10 
. 20 
1 
S 3 
2 
.. .do. 
1 
Js 
Do. 
T .* * 
5. 
. 1 
. 20 
1 
8 | 
H 
.. .do. 
1 
w 
Incomplete. 
6. 
. 1 
•OS 
None. 
. 20 
1 
.. .do. 
1 
Complete. 
. 1 
. 20 
1 
.. .do. 
1 
Do. 
/ . 
ft 
. 1 
. 10 
None. 
d ^ 
cn 
.. .do. 
1 
Do. 
a Similar tests were made with solutions containing 20, 30, 40, and 50 mgm. of tuberculin precipitate in 
10 c. c. of a solution of sodium chlorid. 
& In solutions 1, 2, and 3, four mgm. per cubic centimeter were used. 
* 
