IO 
Journal of Agricultural Research 
Vol. VIII, No. i 
Table V .—Titration for anticomplementary dose 
Tubercu¬ 
lin pre¬ 
cipitate 
solution. 
Bacil¬ 
lary 
emul¬ 
sion. 
Inacti¬ 
vated 
normal 
serum. 
Com¬ 
ple¬ 
ment, 
5 per 
cent. 
Physio¬ 
logical 
salt 
solution. 
Incu¬ 
bation 
period. 
Hemo¬ 
lysin 
(1 unit= 
0.5 c. c.) 
Ery¬ 
thro¬ 
cytes, 
3-5 per 
cent 
Incuba¬ 
tion 
period. 
Degree of 
hemolysis. 
C. c. 
o. I 
. i 
. i 
. i 
. i 
. i 
. i 
. i 
C. c. 
0.40 
•30 
. 20 
* *5 
. 10 
•os 
None. 
. 1 
C. c. 
0.2 
. 2 
. 2 
. 2 
. 2 
. 2 
. 2 
None. 
C. c. 
1 
1 
1 
1 
1 
1 
1 
1 
>> 
'■5 0 
II 
m 
J • 
H 
2 units.. 
.. .do. 
.. .do. 
.. .do. 
...do. 
.. .do. 
.. .do. 
.. .do. 
C. c. 
1 
1 
1 
X 
1 
X 
I 
X 
8 
43 
H 
Incomplete. 
Complete. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Table VI .—Titration for hemolytic properties 
Tube No. 
Solution 
of tuber¬ 
culin pre¬ 
cipitate. 
Bacillary 
emulsion. 
Inacti¬ 
vated 
serum. 
Erythro¬ 
cytes, 3*5 
per cent. 
Physio¬ 
logical 
salt 
solution. 
Incuba¬ 
tion 
period. 
x. 
C. c. 
0.1 
C, c. 
0.40 
•30 
. 20 
C* c. 
C. r. 
1 
£ 
2. 
. 1 
• . 2 
1 
?3 d 
3. 
p d 
• is 
o* ^ 
g 
5... 
II 
II 
3 ** 
6. 
. 1 
•05 
None. 
. 2 
1 
H 
7. 
. 1 
1 
8. 
. 1 
•15 
None. 
None, 
1 
CO 
Degree 
of hemo¬ 
lysis. 
None. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Table VII demonstrates the procedure employed in t he final test and 
also the controls used. 
Table VII .—The final test 
Tube No. 
Antigen. 
Inactivated serum 
0.2 c. c. 
Com¬ 
ple¬ 
ment, 
5 per 
cent. 
Physio¬ 
logical 
salt 
solution. 
Incu¬ 
bation 
period 
at 3 7°C. 
Hemo¬ 
lysin (1 
unit = 
0.5 c.c.). 
Ery¬ 
thro¬ 
cytes, 
3*5 per 
cent 
Incu¬ 
bation 
period 
at 3 7°C. 
Degree of 
hemo¬ 
lysis. 
Posi t i v e 
controls: 
Unit, 
C.c. 
V 
Units. 
C. c. 
1. 
1 
+ Serum. 
1 
O 
' 2 
x 
None. 
2...... 
lV 2 
.do. 
1 
N 
2 
Do. 
3.. 
.do. 
x 
2 
I 
Perfect. 
Negat i v e 
i 
controls: 
0 
4...... 
I 
— serum. 
x 
. 
2 
I 
• 
Do. 
5.. 
lV 2 
.do.. 
I 
$ 
g 
2 
x 
§ 
Do. 
6. 
__do. 
I 
2 
I 
Do. 
Suspected 
H 
serum: 
3 
7 . 
I 
Suspected serum.. 
I 
u 1 
2 
I 
? 
8.. 
.do. 
x 
J a 
2 
x 
? 
9. 
.do. 
I 
•s 
2 
I 
? 
Controls: 
■ s B 
10. 
lY 2 
I 
s 
2 
I 
Perfect. 
ii. 
I 
GQ 
, 
2 
* I 
Do. 
