Jan. 2, 1917 
Diagnosis of Tuberculosis by Complement Fixation 
11 
SOURCE AND TESTING OF SAMPLES 
The material for testing was obtained from animals slaughtered at the 
Baltimore and the Washington slaughterhouses and from animals which 
were subjected to periodical tuberculin tests. The samples obtained 
from the abattoirs were collected in sterile glass jars or test tubes. Every 
sample of blood was accompanied by a post-mortem report from the 
veterinary inspector, describing in detail the condition of the animals 
and also the character and extent of the lesions. Figure 1 is a repro- 
UNITED STATES DEPARTMENT OF AGRICULTURE, M.l. FOftM liar,," 
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REPORT OF FINAL POST-MORTEM EXAMINATION OF RETAINED QffiGASSES. 
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Fig. i.—S pecimen of post-mortem report on retained carcasses in Federal meat inspection 
duction of a post-mortem report showing the character of the lesions of 
animals the serums of which were subjected to the complement-fixation 
test. 
The samples of blood obtained from animals prior to the injection of 
tuberculin were conveyed from the jugular direct into sterile bottles. 
All samples of blood were delivered at the laboratory without any delay. 
After the breaking up of the clots, they were centrifuged; and from the 
supernatant clear serum a sufiicient quantity was taken for the test. 
Some tests were performed with active serums, but without success, 
as the results in most instances showed an anticomplementary action. 
