40 
Journal of Agricultural Research 
Vol. VIII, No. 2 
suitable serum preservative by Voegtlin (n). The following quotation 
from his work (p. 118) is of interest: 
From the experiments described in this bulletin it would seem that chloroform 
when added to serum even to the point of saturation, is not capable of imparting to 
the serum a degree of toxicity which could be compared with that obtained on mixing 
serum with phenol and trikresol (0.25 and 0.5 per cent.). Practically the only effect 
which could be produced by the subdural injection of chloroform serum is the result 
of an increase in intracranial tension. With the use of the gravity method such results 
are not very apt to follow and it seems very doubtful that they occur at all. We, 
therefore, strongly suggest that chloroform be used as a preservative for antimeningitis 
serum. It is well recognized that serum preserved with chloroform after long standing 
will show a cloudiness which is probably due to the partial precipitation of the serum 
proteins. This fact, however, does not alter the efficiency of such a serum in the 
treatment of the disease. 
With one or two exceptions the globulin concentrates were not 
Berkefeld-filtered. For analytic data on the serums and their concen¬ 
trates see Table V. 
Table I contains the data on the preparations of globulin concentrates 
from the serum. The figures in the last column are of particular interest. 
They show that on the average the concentrates from serum 48 contained 
46 per cent, and from serum 96,68 per cent, of the original total coagulable 
protein in the serum used in the preparations. In the inoculation tests 
serum 48 and globulin 48 were generally more potent than serum 96 and 
globulin 96. 
TabIvE I. Data on the preparotions of globulin Concenttotcs ffont setutn 
SERUM 48 
Date when blood 
was drawn. 
Volume 
of serum 
used for 
globulin 
concentra¬ 
tion. 
Heated at 
60 0 C. 
Resultant 
volume of 
globulin 
concen¬ 
trate. 
Total 
coagulable 
protein 
in serum 
used. 
Total co¬ 
agulable 
protein in 
concen¬ 
trate. 
Total protein in con¬ 
centrate. 
July 26, 1915.. .. 
Sept. 21, 1915... 
Do... 
C. e. 
1, OOO 
650 
650 
900 
900 
700 
700 
Hours. 
(a) 
O 
6 
0 
6 
0 
6 
C. c . 
220 
190 
115 
220 
24O 
92 
95 
Gut . 
81. 7 
49. 8 
49. 8 
61. 2 
61. 2 
47 * 2 
47*2 
Gm. 
38.6 
27. O 
15*7 
33*4 
33*6 
19.4 
20. 0 
Per cent. 
47 ' 
. 54 
32 
55 [Average, 46 
55 
41 
42 , 
Nov. 5, 1915. . .. 
Do . 
Jan. 6 , 1916'..... 
Do . 
SERUM 96 
July 26, 1915. 
Sept. 21, 1915.... 
Do. 
Nov. 5, 1915- 
Do. 
Jan. 6, 1916. 
Do. 
1, 200 
(a) 
460 
97.8 
78.3 
80^ 
650 
0 
255 
55*9 
36. 8 
66 
650 
3 
235 
55*9 
33*2 
59 
900 
0 
404 
79.8 
59*5 
74 
Average, 68 
900 
6 
400 
79.8 
60. 1 
75 
700 
0 
146 
55*2 
32. 8 
59 
700 
6 
158 
55*2 
34 - 6 
■ 63J 
0 Heated in water bath at 60 0 C. until temperature inside of flasks was very near 6o°. 
