jan. 8 , 1917 Immunity Studies on Anthrax Serum 43 
sponding unheated preparation. Just why heat should be detrimental 
to one serum and apparently without effect on the other is difficult to say. 
It is possible that serum 48, being the more potent of the two, lost only a 
relatively small part of its total potency when heated; while serum 96, 
being comparatively weak, lost a relatively large part of its potency under 
the same treatment. At the present state of our knowledge there seems 
to be no need for the heat treatment, although future work may indicate 
its desirability. 
Table II .—Results of test 5 at 72 and 144 hours after the inoculation of guinea pigs 
with virus C. Seventy-two guinea pigs were inoculated with serum and globulin on 
Dec . 11,1915, and with 0.25 c. c. of virus C on Dec . 13,1913 . Six additional guinea 
pigs {controls) received only virus a 
Serum 48. 
Globulin 48. 
Dose. 
Globu¬ 
lin in 
dose. 
Result. 
Not heated. 
Heated. 
hours. 
144 
hours. 
Dose. 
Globu¬ 
lin in 
dose. 
Result. 
Dose. 
Globu- 
linin 
dose. 
Result. 
hours. 
144 
hours. 
hours. 
„ i 44 
hours. 
C. c. 
Gm. 
C. c. 
Gm. 
C. c. 
Gm. 
Tliprl 
Died... 
Died... 
1 
0.044 
Lived.. 
Lived. 
0.25 
0.038 
Lived.. 
Died. 
0.25 
0.03S 
Lived.. 
Died. 
..do... 
Do. 
. .do. .. 
Do. 
. .do. .. 
Do. 
dr» 
Died. 
Died... 
2 
.088 
Lived.. 
Died. 
• 5 ° 
.076 
. .do.. . 
Lived. 
*50 
.070 
Lived.. 
Lived. 
. .do. .. 
Do. 
. .do. . . 
Died. 
. .do. .. 
Do. 
[..do... 
Do. 
..do... 
Lived. 
. .do. .. 
Died. 
3 
• 131 
. .do... 
Do. 
• 75 
.114 
..do... 
Do. 
•75 
.105 
..do. .. 
Do. 
..do... 
Do. 
..do... 
Do. 
.. .do. .. 
Lived. 
Do 
Died .. 
..do... 
Died. 
4 
. 17s 
. .do... 
Lived. 
1.00 
.152 
Lived.. 
Died. 
1.00 
. 140 
..do. .. 
Do. 
..do... 
Do. 
.. .do. .. 
Lived. 
L.do... 
Lived. 
Serum 96. 
Globulin 96. 
C. c. 
Gm. 
C. c. 
Gm. 
C. c. 
Gm. 
Died 
Died 
(Died. .. 
1 
0.075 
..do... 
Lived. 
0.25 
0.037 
do. 
......... 
0.25 
0.037 
\ ..do... 
......... 
..do... 
Do. 
Lived.. 
Died. 
1..do... 
.. 
Died... 
[..do... 
2 
.151 
< ..do. .. 
. 
Lived. 
• 5 © 
.074 
. .do. .. 
. 
•50 
•075 
\. .do... 
. 
. .do. .. 
Do. 
Lived.. 
Died. 
1. .do. .. 
Died... 
f..do... 
3 
.226 
< Lived.. 
Lived. 
*75 
. hi 
. .do. .. 
......... 
•75 
. 1X2 
■{..do. .. 
. 
..do... 
Do. 
. .do. .. 
. 
L .do... 
. 
..do... 
Do. 
..do... 
. 
f..do... 
. 
4 
.302 
..do... 
Do. 
1.00 
.147 
..do... 
. 
1.00 
• ISO 
■{. .do. .. 
. 
1. .do... 
Do. 
1. .do. .. 
. 
(.Lived.. 
Died. 
a The six control guinea pigs died in less than 72 hours. 
Globulins were prepared from blood drawn on November 5, 1915. 
In serums 48 and 96 the globulin consisted largely of pseudoglobulin; the globulin preparations con¬ 
tained only pseudoglobulin (see page 39). 
In making the heated preparations the serum was heated for six hours at 60 C. 
If Tables II and III be divided horizontally by a line running between 
the 2 and 3 c. c. doses of serum, and the number of survivals in the upper 
half compared with those in the lower, it will be apparent that the pro¬ 
tective action of neither serum nor globulin preparations was strictly 
proportional to the dose. In Table II there were 10 survivals in the upper 
67905°—17 - 2 
