44 
Journal of Agricultural Research 
Vol. VIII, No. 2 
half and 13 m the lower. In Table III there were 18 survivals in the upper 
and 17 in the lower. These results indicate that the dose is only one 
of the many factors which decide the course of an individual test. They 
also indicate the desirability of large numbers of results before generali¬ 
zations are made. 
Table III .—Results of test 7 at 72 and 144 hours after the inoculation of guinea pigs with 
virus D. Seventy-two guinea pigs were inoculated with serum and globulin on Jan. 15, 
1916, and with 0.25 c. c. of vifus D on January 17,1916 . Six additional guinea pigs 
(controls) received only virus a 
Serum 48. 
Globulin 48. 
Result. 
Not heated. 
Heated. 
Dose. 
Globulin 
in dose. 
144 
hours. 
Globulin 
in dose. 
Result. 
Globulin 
in dose. 
Result. 
. 7 * 
hours. 
Dose. 
hours. 
144 
hours. 
Dose. 
hours! 
144 
hours. 
C.c. 
X 
Gm. 
0.044 
[Lived. 
..do... 
. .do... 
..do... 
..do... 
..do... 
..do.,. 
..do... 
..do... 
..do... 
, .do... 
..do... 
Died. 
Lived. 
Do. 
Died. 
Lived. 
Do. 
Died. 
Lived. 
Do. 
Do. 
Do. 
Do. 
C.c. 
0.25 
Cm. 
0.038 
[Died.. 
< Lived. 
I..do... 
rni*vi 
Died. 
Lived. 
C.c. 
0.25 
Gm. 
0-035 
Lived. 
..do... 
..do... 
Died.. 
Died. 
Lived. 
Do. 
2 
.088 
*50 
.076 
< Lived. 
|..do... 
f. .do... 
Died. 
Lived. 
Died. 
•50 
.070 
Lived. 
..do... 
Died.. 
Died. 
Do. 
3 
.131 
•75 
.1x4 
h.do... 
|..do... 
[Died.. 
Do. 
Lived. 
•75 
.105 
Lived. 
Died . 
Lived. 
Lived. 
Died. 
4 
♦ 
• *75 
1.00 
.15* 
\..do... 
1 Lived. 
Lived. 
x. 00 
. 140 
..do,.. 
..do... 
Lived. 
Do. 
Serum 96. 
Globulin 96. 
C. c. 
X 
Grin. 
0.075 
Died.. 
Lived. 
..do... 
..do... 
..do... 
..do... 
C.c . 
Gm. 
0.074 
Lived. 
. .do... 
..do... 
T)i «1 
Died. 
C. c. 
Gm. 
[Died.. 
Lived. 
Do. 
Do. 
Do. 
Do. 
0. so 
Lived. 
Do. 
0.50 
0.075 
..do... 
Lived. 
. .do... 
Died. 
Do. 
2 
• 151 
x.oo 
.147 
..do... 
Lived. 
[Died.. 
Lived. 
x.oo 
.150 
..do... 
..do... 
Died.. 
Lived. 
Do. 
3 
.226 
Lived. 
..do... 
..do... 
..do... 
..do... 
Died. 
Lived. 
Died. 
Lived. 
Do. 
x.50 
.221 
Lived. 
,..do... 
. .do.. 
Lived. 
Do. 
Died. 
1.50 
• 225 
..do... 
Lived. 
[Died.. 
Lived. 
4 
.302 
2.00 
.294 
..do... 
..do... 
Do. 
Do. 
2.00 
.300 
Lived. 
..do... 
Died. 
Lived. 
a Of the 6 control guinea pigs, 3 died in less than 72 hours and the remaining 3 died in less than 144 hours. 
Globulins prepared from blood drawn Nov. s, 1915 - 
In serums 48 and 96 the globulin consisted largely of pseudoglobulin; the globulin preparations contained 
only pseudoglobulin (see p. 39)- . , a 
In making the heated preparations the serum was heated for 6 hours at 60 C. 
In test 6 the protective action of the globulin preparations on horses, 
calves, and sheep was demonstrated. The results are contained in 
Table IV. 
All of the other globulin preparations were found to be potent when 
tested. For the reasons already stated, the protective power of the 
different preparations can be measured only approximately, and therefore 
the influence of heat, the relation between globulin contents and potency, 
etc., will remain in the problematic stage until standardized units of 
anthrax toxin and antitoxin are available for experimental purposes. 
