Jan. 8,19x7 
Immunity Studies on Anthrax Serum 
45 
Table IV .—Test 6 : Results of inoculations of larger animals. The animals were inoculated 
with globulin on Dec. 17, 1915 , and with virus “Chestertown” on Dec. 21 , igi$ a 
Animal No. 
Dose of globulin. 
Pseudo¬ 
globulin 
in dose. 
Dose of 
virus. 
Result. 
Gm. 
C. c. 
5 c. c. globulin 48. 
0. 760 
0. 5 
Alive, Jan. 4, 1916. 
Horse 130. 
5 c. c. globulin 48 (heated).... 
. 700 
*5 
Do. 
8 c. c. globulin 96. 
1.179 
. 5 
Dead, Dec. 30, 1915. 
8 c. c. globulin 96 (heated). 
x. 202 
. 5 
Dead, Dec. 31,1915. 
Control. 
. 5 
Dead, Dec. 27,191s. 
Calf 86 . 
S c. c. globulin 48. 
. 760 
. 5 
Alive, Jan. 4, 19x6, 
5 c. c. globulin 48 (heated). 
. 700 
.5 
Do. 
87 . 
8 e. c. globulin 96. 
x. 179 
. 5 
Do. 
Calf 69. 
8 c, c. globulin 96 (heated). 
X. 202 
. 5 
Do. 
Caff -1 . 
Control. 
. 5 
Do. 
5 c. c. globulin 48. 
. 760 
. 25 
Dead. Dec. 30, 1915. 
5 c. c. globulin 48 (heated). 
. 700 
• 25 
Alive, Jan. 4, 1916.* 
8 c. c. globulin 96. 
I. 179 
* 25 
Dead, Dec. 23,1915. 
8 c. c. globulin 96 (heated). 
X. 202 
• 25 
Do. 
Control. 
•2S 
Do. 
a In makin g the heated globulin preparations the serum was heated 6 hours at 6o° C. The globulins 
were prepared from blood drawn November 5, 1915* 
During a recent outbreak of anthrax in a herd of animals near Rich¬ 
mond, Va., anthrax-globulin preparations were used with very good 
results. In the course of a week several cows had been lost on this farm 
and others were sick. An investigation showed the presence of anthrax 
infection. At this time three cows had high temperatures (io4°-io6° 
F.) and were manifesting severe symptoms of the disease. Twenty c. c. 
of globulin prepared from serum 48 were administered intravenously to 
each of the three animals. One of the animals was in a dying condition 
at the time the injection was made and died shortly afterwards. The 
next morning a decided drop in temperature was noted in the two other 
animals. Another injection of 20 c. c. of globulin was administered that 
afternoon. Complete recovery resulted in both cases. Prophylactic 
treatment was given to 244 head of cattle and 25 horses and mules, con¬ 
sisting of injections of 6 c. c. of globulin where that prepared from serum 
96 was used, and 4 c. c. of the serum 48 preparation, administered simul¬ 
taneously with 1 c. c. of a standardized anthrax-spore vaccine. Up to 
the present time no additional losses from anthrax have been reported in 
this herd. 
CHEMICAL ANALYSES OF SERUM AND GLOBULIN PREPARATIONS 
The first few analyses of serum and globulin were, made by the methods 
described by Banzhaf and Gibson (3). These methods were found to be 
extremely laborious. If, after precipitating globulin by one-half satura¬ 
tion ammonium sulphate, the mixture was filtered through ordinary ash¬ 
less paper, the filtrates were cloudy, and a sharp separation of precipitate 
from filtrate was uncertain. If filtered on hard paper (S. & S. 575), the 
filtrates were clear, but filtration was extremely slow and evaporation 
