Jan. 8,19x7 
Immunity Studies on Anthrax Serum 
49 
correct result are recorded because they indicate, to some extent, just 
how the results are affected by the differences in technic. For most 
purposes it is probable that any method will yield results that are com¬ 
parable and useful. One of the objects of the repeated trials was to 
develop a technic by which it would be possible accurately to determine 
euglobulin, pseudoglobulin, and albumin separately in any one sample of 
serum, so that the sum of the three determinations would almost exactly 
equal the total coagulable protein in the same serum. Theoretically 
this should be true; practically the numerous sources of unavoidable 
error do not permit such accurate work. 
Table V .—Analytic data on serums and globulin preparations 
Date blood was drawn. 
In 10 c. c. of— 
Total coagulable 
protein. 
Total 
Total 
globulin 
Serum. 
Globu¬ 
lin.® 
globulin.® 
in total 
protein. 
Serum 48. 
Gm. 
0.817 
Gm. 
Gm. 
6 0.643 
Per cent . 
79 
Do. 
Globulin 48. 
x -757 
Sept. 21, 1015.. 
Serum 48. 
.767 
c - 5°3 
66 
Do... 
Globulin 48. 
x. 420 
1-365 
Do... 
Globulin 48 (heated).... 
Nov. 5, 1915. 
Serum 48. 
.680 
y 438 
*•457 
64 
<*.672 
Do. 
Globulin 48. 
1. 520 
1.400 
Do. 
Globulin 48 (heated).... 
Jan. 6, 1016. 
Serum 48... 
.674 
.411 
61 
Do.■. 
Globulin 48. 
Do. 
Globulin 48 (heated).... 
2. XIO 
Tiilv 26. IOI<. 
Serum 96... 
.815 
6.649 
80 
Do. 
Globulin 96. 
1.704 
Sept. 2i, 1915. 
Serum 96... 
.861 
c.669 
78 
‘Do/.. 
Globulin 96. 
1-445 
I- 4 I 5 
Do. 
Globulin 96 (heated).... 
Nov, 5,1915. 
Serum 96.. 
.887 
d. 893 
- 754 
d.744 
85 
Do. 
Globulin 96. 
Do. 
Globulin 96 (heated).... 
1.502 
Jan. 6,1916. 
Serum 96. 
.789 
. 627 
79 
Do.^. 
Globulin 96. 
2.247 
2.193 
Do. 
filnhiilin 96 (tipatpH) 
Aug. i6 f 1915.. 
Serum 148. 
• 75 * 
•645 
•734 
.768 
.768 
<*.776 
1.047 
.907 
•750 
•673 
•793 
•745 
• 730 
c - 4*9 
c . 3*2 
*• 331 
/• 333 
• 35 ® 
6.384 
-438 
•455 
• 455 
d. 436 
.796 
.644 
*.461 
6.369 
4340 
f- 337 
/. 366 
.■414 
7.492 
•533 
•494 
.4x4 
56 
Oct, s, 1915.... 
.do... 
Oct. 25,1915. 
55 
Nov. 19,1915. 
60 
59 
59 
Dec. is. iqis.'. 
Feb. 2,1916. 
76 
71 
61 
Mar. 6,1916... 
Aug. 16, X915. 
Serum 149. 
Oct. s, 1915. 
Oct. 25,1915.. 
62 
Nov. 19,1915... 
.do. 
67 
66 
Dec. 15, 1915... 
57 
a All determinations of globulin are by single precipitation and centrifuging unless otherwise Qualified. 
6 Globulin precipitated once and filtered. 
c Globulin precipitated twice and filtered. 
d Repetition of previous determination. 
e Globulin by magnesium-sulphate saturation, twice precipitated and filtered. 
1 Globulin precipitated twice and centrifuged. 
