52 
Journal of Agricultural Research 
V6I. VIII, No. s 
The term “total globulin” is here used, as it is in Table V, to include 
the protein precipitated on 50 per cent saturation with ammonium sul¬ 
phate. in so far as mule 148 received increasing doses of virus during 
the immunization, the statement may be made that with the rise in 
total protein and globulin there was a rise in potency; or at least the 
resistance of the mule was tremendously increased toward anthrax virus. 
It would be unsafe to generalize from the curve for a single animal, 
but obviously in the case of mule 148 the presence of a soluble toxin 
was not necessary for the production of those serum protein changes 
usually noticed in diphtheria- and tetanus-immune serums. 
Similar changes were observed by Hartley (5, p. 268) in immuniza¬ 
tion against rinderpest. The increase in total protein and total globulin 
during immunization has therefore been observed under the following 
five conditions: 
(1) In diphtheria, in which a soluble toxin is involved. 
(2) In tetanus, in which a soluble toxin is involved. 
(3) In anthrax, in which no soluble toxin is involved according to the 
present state of our knowledge. 
(4) In rinderpest, in which a filterable virus is involved. 
(5) In diphtheria, when there is an increase of serum proteins without 
the production of antibodies. 
TREATMENT OF ANTHRAX IN MAN 
Since the beginning of the preparation of anthrax serum and globulin 
by the Bureau of Animal Industry, considerable amounts of these prod¬ 
ucts have been furnished for the treatment of the disease in man, with 
highly satisfactory results. 
Of the serum, the curative dose recommended is 40 c. c. administered 
subcutaneously in four or five places, repeated as necessary, after inter¬ 
vals of 24 hours, with injections of 25 c. c. In advanced cases the dose 
may be increased and the injections made intravenously. The dose of 
the globulin preparation is based on its proportional concentration and 
is usually from 10 to 15 c. c. The dose being considerably smaller than 
that of serum, the injection may be safely made intravenously. With 
other types of protein absent the globulin preparation is superior to the 
serum in that anaphylactic reaction is minimized. 
A number of cases of anthrax in man were treated at Bellevue Hospital, 
New York, N. Y. Reports from this institution on several of these cases 
show that a marked lowering of the temperature and reduction of the 
edematous swelling followed the first injection of serum. 
STANDARDIZATION OF ANTHRAX SERUM BY COMPLEMENT FIXATION 
It has been found that the serum from various animals treated in an 
identical manner varies greatly in.potency, so that one of the chief difficul¬ 
ties in the production of anthrax serum is its standardization, the various 
