140 Journal of Agricultural Research voi. vm, No. 4 
Ehrbg. He grew the fungi on gelatin in petri plates at temperatures of 
o°, 4.5 0 , 9.5 0 ,14 0 , and 18 0 G. At the end of 35 days all but the last two 
of these fungi had made a measurable growth at o°, Botrytis cinerea 
making the most vigorous growth. At 4.5 0 Rhizopus nigricans and 
Gloeosporium fructigenum had produced small colonies at the end of 35 
days. 
Penicillium glaucum , Botrytis cinerea , Monilia fructigena, and Gloeo¬ 
sporium album were inoculated into apples and the fruit stored in one case 
at 4.5 0 and in another at 14 0 C. The diameter of the rots produced at 
the end of two and three weeks is given. The first three fungi made a 
fair growth at the lower temperature. 
In a later paper Schneider-Orelli 1 reported temperature studies on 
different species or strains of Gloeosporium fructigenum. He found that 
the European form had lower optimum, maximum, and minimum tem¬ 
peratures than the American form. At 5 0 C. the European form, when 
grown in gelatin in petri plates, produced a colony 0.4 cm. in diameter 
in 12 days and a colony 3.7 cm. in diameter in 35 days, while the Ameri¬ 
can form had made no growth at the end of 35 days. 
Edgerton 2 made a study of the behavior of various American species 
and strains of Glomerella. He found that there were two different strains 
of Glomerella on the apple, a rapid-growing southern form and a slow- 
growing northern form. The former had an optimum temperature of 
27 0 to 29 0 C. and a maximum temperature above 37.5 0 , produced scanty 
aerial mycelium in culture, formed cankers on apple limbs, besides rotting 
the fruit, and very commonly produced perithecia on the host and in 
culture. The latter had an optimum temperature of 24 0 to 25 0 and a 
maximum temperature of 34 0 to 35 °, produced a greater abundance of 
white aerial mycelium in culture, and apparently did not produce cankers 
on apple limbs, though it rotted the fruit. He thought the former should 
be called “ Glomerella cingulata )y and that the latter should retain the 
name li Gloeosporium fructigenum” given by Berkeley. He considered 
the latter fungus probably identical with the European one. 
Ames 3 determined the germination and growth of Monilia fructigena , 
Penicillium digitatum (Fr.) Sace., Rhizopus nigricans Ehrenb., Glomerella 
rufomaculans , and Cephalothecium roseum at various temperatures. The 
germination tests were made by means of Van Tieghem cell drop cultures. 
At i° C. only the first two of these fungi germinated within 245 days, 
while at 3 0 to 4 0 all but Cephalothecium roseum germinated within 200 
days. The growth of the fungi was tested on bean agar, and in this series 
Penicillium glaucum was substituted for P. digitatum . None of the fungi 
except P. glaucum made any growth at a temperature of i° to 2 0 and 
1 Schneider-Orelli, Otto. Op. cit., 1912. 
2 Edgerton, C. W. The physiology and development of some anthracnoses. In Bot. Gaz., v. 45, no. 6, 
P* 393. 402-403. 1908. 
- Effect of temperature on Glomerella, In Phytopathology, v. 5, no. 5, p. 247-259, 4 fig. 1915. 
3 Ames, Adeline. The temperature relations of some fungi causing storage rots. In Phytopathology, v, 
5, no. 1, p. 11-19. 1915* 
