Feb. 5, 1917 
Leafspot-Rot 0} Pond Lilies 
221 
205. The same fungus was also obtained in pure culture from pieces of 
the diseased leaf tissue. Three species of bacteria, one giving yellow 
and two giving white colonies, a sterile nonseptate fungus, and species 
of Aspergillus, Penicillium, and Botrytis were also obtained in culture by 
this method. 
July 20-22, 1914.—Leaves of N. odorata with early stages of infection 
were collected in an open pond at the Brooklyn Botanical Gardens, 
Brooklyn, N. Y.; and two days later similar specimens were collected 
at Arlington, N. J. These were taken to the laboratory and plate cul¬ 
tures started from bits of diseased leaf tissue after sterilizing and washing 
the surface of the leaves. In this way a fungus similar to isolation 205 
was obtained from each set of material. The fungus obtained from the 
Arlington material was designated as isolation 217 and that from the 
Brooklyn material as isolation 220. 
August 24, 1914.—Affected leaves of one of the blue lilies were col¬ 
lected from an open pond at Kenilworth, D. C. Isolation by the method 
described in the preceding paragraph gave a fungus similar to isolation 
205, which was designated as isolation 225. 
October 30, 1914.—In the same way this fungus was reisolated from 
affected leaves of A. odorata inoculated 01% October 24, 1914, from a pure 
culture of isolation 225. This reisolation was designated as isolation 249. 
The fungus was isolated in the same way by Mrs. Ella M. A. Enlows 
from affected leaves of A. odorata collected at Kenilworth, D. C. (Isola¬ 
tion En 59, September 6, 1915); and from similar leaves collected at the 
New York Botanical Garden, New York City (Isolation En 172, July 
25, 1916). 
The fungus was most readily isolated from young spots from the thicker 
leaved species of pond lily. In those with thin leaves various accom¬ 
panying fungi and bacteria were more abundant, due, probably, to the 
more rapid softening of the tissues and earlier entrance of saprophytes. 
INOCULATION EXPERIMENTS 
Inoculation tests were made with all these isolations upon healthy 
leaves of both hardy and tender species of water lily. A part of these 
tests was made in the laboratory on cut leaves floated in tap water. 
Tiny spore clusters from com-meal-agar cultures were scattered about 
in drops of water on the upper surface of each leaf without abrasion of 
the epidermis and the glass containers were left covered for a few days to 
give a moist condition for the germination of the conidia. Under these 
conditions the leaves are nearly in their normal environment except that 
the leaf petioles are severed from the parent plant. When not inoculated, 
these leaves have remained green and turgid for 10 days to 2 weeks or 
longer. 
Unless otherwise stated, leaves for laboratory inoculation were 
collected from the open ponds at Kenilworth, D. C. Conidia used were 
from 1- to 2-weeks-old corn-meal-agar cultures, and the 10-inch glass 
