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Journal of Agricultural Research voi. viii, No. 6 
containers in which the leaves were floated were left covered for three 
days after inoculation. Control leaves were treated like the inoculated 
leaves, except that the fungus was not introduced. 
Greenhouse inoculations were made upon plants growing in galvanized- 
iron tubs in one of the department greenhouses. These inoculations 
were carried out in the same way as in the laboratory. Each leaf was 
left covered for several days with a shallow bell jar supported on stilts 
so that the base of the bell jar dipped just below the surface of the 
water. Details of the inoculation experiments are given below. 
Laboratory, June 14, 1913. Isolation 205.—Twelve leaves of N. odorata were 
inoculated on the upper surface, and four leaves, floated upside down, were inoculated 
on the morphologically lower surface. Four control leaves were not inoculated. At 
the same time two leaves each were inoculated with the following organisms isolated 
from diseased leaves: Aspergillus sp., Penicillium sp., Botrytis sp., a sterile nonseptate 
fungus, and the three types of bacteria. Two days later tiny dark-reddish specks 
were observed on the leaves in every case where the upper surface had been inoculated 
with isolation 205, except where parts of the surface of the leaves were several milli¬ 
meters below the surface of the water. In the latter cases no signs of infection were 
evident. Leaves inoculated on the lower surfaces, those inoculated with other fungi 
and bacteria, and the control leaves showed no signs of infection throughout the 
experiment. . 
After four days the infected areas were 3 to 4 mm. in diameter, the centers brownish, 
and the outer parts dark olivaceous black. Deeply submerged parts of leaves were 
still unharmed by the presence of the fungus. The leaves inoculated on the lower 
surface were mostly submerged, and no visible infection had occurred, except at a 
point in one leaf near the surface of the water where a break had occurred in the 
epidermis. The experiment was run for 10 days with no further change except an 
increase in size of infection areas until at the close, when the infected leaves were for 
the most part a mass of decaying tissue. The tests with the other forms of fungi and 
bacteria were repeated twice and in every case gave a negative result, so that these 
organisms were discarded and attention devoted henceforth to the form represented 
by isolation 205. 
Laboratory, June 19, 1914. Isolation 205.—Eight leaves of N. odorata were 
inoculated with this isolation. After two days infection had started at the inoculation 
points. After five days the spots were 3 to 35 mm. in diameter. Each inoculation 
had taken, and the three controls were perfectly sound. 
Laboratory, July 7,1914. Isolation 205.—Inoculations were made on 12 leaves 
of N. odorata, 6 leaves of N. caerulea (PI. 68, A, B), 6 leaves of N. tuberosa (PI. 67, A), 
and 3 leaves of one of the tender day-blooming blue lilies. After three days tiny infec¬ 
tion specks were observed at nearly every spot inoculated. After four days infection 
had started at all inoculation points. In most cases there were small reddish spots up 
to the size of a pin head. After a week single spots had increased in area up to 30 and 
35 mm. in diameter. The main part of the diseased spots was water-soaked and 
olivaceous black in color, and in many cases the margins were reddish. No controls 
were run with this test. 
Laboratory, August 8, 1914. Isolations 205 and 227.—One leaf each of N. 
odorata and N. daubeniana was inoculated with isolation 205, and one leaf of each 
species was held for a control. After four days small reddish spots were visible at the 
inoculation points on both leaves. After six days the spots had increased considerably 
in size, and were olivaceous black and water-logged. The controls were sound. 
By the same method, inoculations with isolation 205 were also made on four 
healthy leaves of Egyptian lotus ( Nelumbium speciosum). In this case the glass con- 
