224 
Journal of Agricultural Research 
Vol. VIII, No. 6 
and, so far as could be seen, infection had occurred at every inoculation point. In 
the case of leaves with red pigment on the lower surface there was a tendency toward 
a slight reddish border around the spots as seen from the upper surface. 
Laboratory, November io, 1914. Isolations 205, 225, and 249.—Inoculations 
were made with isolation 205 on five leaves and with isolations 225 and 249 on two 
leaves each of N. odorata , collected from plants growing in one of the department 
greenhouses. Typical infection spots were visible in three days on all inoculated 
leaves. After four days infection areas were evident so far as could be determined at 
every inoculation point, and these varied in diameter from the size of a pin head up 
to 20 mm. in diameter. The five control leaves of the same species remained sound. 
Laboratory, December 31, 1914. Isolations 205 and 225.—Inoculations were 
made with each isolation on five leaves of N. capensis (PI. 68, C) and five leaves 
were held as controls. These leaves were collected from plants growing in one of the 
department greenhouses. After four days infections were starting at every inoculation 
point and after one week the olivaceous-black spots varied from mere specks to spots 
20 mm. in diameter. The lighter green halo surrounding the dead area, as noted 
under a previous test, was much in evidence here. This halo was most prominent in 
the thinner leaved species of pond lily. The five control leaves remained sound. 
Laboratory, April 16, 1915. Isolation 205.—Four leaves of N. capensis , one of 
N. zanzibariensis , and two of N . caerulea were inoculated from a single 2-months-old 
com-meal-agar culture. After three days very numerous pinhead spots were observed 
on all three species of lily, and several olivaceous-black spots up to 5 mm. in diameter 
on N. capensis. After 5 days some of the leaves of N. capensis were a mass of water- 
soaked decaying tissue. . Large water-soaked areas and numerous small spots occurred 
on leaves of the other two species. The light-green halo was noted around the de¬ 
cayed spots on all three species. The four control leaves showed no signs of infec¬ 
tion except that one of them had a small spot at the beginning of the experiment 
and this continued to develop. Apparently it was a natural infection with the same 
fungus. 
Laboratory, June 25, 1915. Isolation 225. Inoculated by Mrs. Ella M, 
Enlows. —One leaf each of N. odorata , and N. zanzibariensis was inoculated with this 
isolation. After three days small infection areas from mere specks up to 3 mm. in 
diameter were observed. The decay progressed rapidly so that after one week 
the two leaves were a mass of soft-rotted tissue. The three control leaves remained 
sound. 
Laboratory, November 17,1915. Isolations 205, and En 59, using 4-day-old 
corn-meal-agar cultures. —Inoculations were made with 205 on one leaf each of 
N. capensis and N. omarana; and with En 59 on one leaf of N. capensis and two leaves 
of N. omarana. After two days tiny infection specks were noted at the points inocu¬ 
lated with En 59 on leaves of N. omarana. After four days infections had started on 
all leaves inoculated with each isolation, and after six days the olivaceous-black 
infection areas from En 59 had in many cases a diameter of 12 to 15 mm., and those 
from 205 in no case were more than 5 mm. in diameter. A-larger number of infection 
areas had resulted and increase in size of spots was more rapid with isolation 
En 59 than with 205. Isolation 205 had been carried in culture for over two years, 
while En 59 was a fresh isolation. Possibly this may account for the difference in 
virulence. 
Laboratory, December 13, 1915. Isolation En 59.—Sclerotia from a 2-weeks- 
old corn-meal-agar slant were used for inoculating three leaves of N. omarana and 
conidia from the same lot of cultures for inoculating two leaves each of N. zanzibariensis 
and N. omarana and three leaves of N. capensis. After two days tiny infection spots 
were noted on the leaves inoculated with conidia, but none on those inoculated with 
sclerotia. After three days small infection spots were just visible on the three leaves 
inoculated with sclerotia, while they were as large as 2 mm. in diameter on the 
