324 
Journal of Agricultural Research 
Vol. VIII, No. 9 
spores. The spores are ovoidal or elongated rarely inequilateral and of 
the same color as the conidia. They are somewhat larger than the 
conidia, being i8.to 30 by 10 to 15 /z. They lack the striations which 
characterize the conidia, but are unique in that they are provided with 
two or three terminal or lateral hyalin appendages, from each of which 
a tuft of 12 to 20 hairlike processes extend (Pi. 85, N). These ciliate 
processes are from 1 to times as long as the spores. 
GERMINATION OF SPORANGIOSPORES 
The germination of these spores is quite similar to that of conidia 
Within two or three hours after the spores are placed in nutrient media 
there is an increase in size of the spores, followed by the emergence of 
one or two lateral germ tubes (Pi. 85, /). Within a few hours a pro¬ 
fusely branched mycelium is formed, which in 24 to 48 hours has ma¬ 
tured a crop of sporangia and conidia. 
CHEAMYDOSPORES 
The mycelim in 4- to 5-day-old cultures on nutrient agar is commonly 
septate. Not infrequently well differentiated chlamydospores, as shown 
in Plate 85, 0 , will have formed within this time. In cultures 2 weeks 
old chains of chlamydospores, as represented in Plate 85, M, are some¬ 
times developed. No chlamydospore formation occurred in the form 
which Thaxter 1 had under observation. These bodies have never been 
observed to germinate. 
ZYGOSPORIC ■ STAGE 
Cunningham observed zygospore formation in the two Indian species 
of Choanephora, and his account of the development of CAnfundibtUifera 2 
contains a detailed description of this process. Zygospores of C. cucur - 
bitarum appear not to have been observed prior to the present study. 
No noteworthy differences are presented by the American species when 
contrasted with the Indian forms. A club-shaped branch first arises 
from each of two adjacent mycelial filaments. These gradually enlarge 
and become densely filled with granular protoplasm. The contents tend 
to accumulate in the apical extremity, and a septum forms, separating 
the gamete from the suspensor. When two of these branches are sit¬ 
uated sufficiently close together, their bowed tips come in contact and 
the gametes become flattened along the opposed surfaces. The parti¬ 
tion is then absorbed and the two gametes fuse. Such a stage is repre¬ 
sented in Plate 86, B , C, at which time the two saccular suspensors are 
quite clear and empty as contrasted with the immature zygospore, which 
is convex on the upper side and is filled with coarse granules and nu¬ 
merous yellowish oil globules. As the zygospore matures, this convexity 
1 Thaxter, Roland. Op. cit. 
2 Cunningham, D. D. Op. cit. 
