Mar. 6 , 1917 
Little-Leaf of the Vine 
395 
Injections of iron-sulphate solutions (i: 500) and of copper-sulphate 
solutions (1:500 and 1:1,000) were made on a number of vines. The 
solutions were caused to enter the vine by means of a J^-inch hole bored 
halfway, through the trunk near the surface of the ground. A metal 
tube was inserted into this hole and connected, by means of a rubber tube, 
to a funnel fastened to the head of the vine. The solution was poured 
into the funnel and was absorbed very readily. From 250 to 375 c. c. 
were used on each vine. These injections were made at various times 
from June 9 to September 10 in 1910. No results were obtained with 
the iron sulphate. 
The copper sulphate produced some remarkable effects*. One vine 
which received an injection of 375 c. c. of a 1 to 500 solution of copper 
sulphate on June 9 lost all its leaves in a few days, but a new growth 
started almost immediately, and by July 15 the vine was covered with a 
full supply of apparently healthy and normal foliage. A neighboring 
vine showing about the same degree of disease at the time of the injec¬ 
tion was left untreated, and on July 15 had shown no signs of recovery 
(PI. 92, A , a).- All vines (11) which received injections of copper sulphate 
reacted in the same way. The first injury to the leaves and their sub¬ 
sequent recovery, however, were not so marked as with the first vine 
treated. The later the treatment, the smaller the effect. In all cases, 
however, the treated vines could readily be picked out by their more 
abundant, greener, and healthier looking foliage. 
Examinations of the treated vines during the following year showed 
that the improvement had not been permanent. There was no ap¬ 
parent difference between the treated and the untreated vines when 
examined in 1911. 
Similar injections were made with a larger number of vines during 
the following year in winter, spring, and in June. The winter injections 
apparently increased the disease. Those of spring and early summer 
had little effect, though some of those made in June improved the vines 
slightly. The vines treated this year were grafted, younger, less badly 
affected, and of a different variety. 
CUI/TURAI, measures 
The effect of fertilization was tried on about one-fifth of an acre in a 
patch of diseased vines. A complete fertilizer recommended by Prof. 
Burd was used at the rate of 400 pounds per acre. It had the following 
composition: 
Superphosphate. 
Do. 
10. 00 
Sodium nitrate. 
.N. 
... 1.50 
Ammonium sulphate.. 
.N. 
Blood. 
.N. 
. .. 2. 50 
5.00 
Potassium sulphate. 
• 
.k 2 o. 
4. 00 
