Mar. is, 1917 Spore-Forming Bacteria of the Apiary 4.1 3 
the organism isolated from sample 2329, the strain of B. vulgatus secured 
from the Bureau of Animal Industry, and the strain of B. vidgatus 
isolated from potato. 
MEDIA USED FOR GROWING THE CULTURES 
Cultures were grown upon agar slopes prepared from both bee-larvae 
bouillon and plain peptonized beef bouillon. The ordinary agar was 
prepared as usual by adding 1.25 to 1.50 per cent of shredded agar to beef 
bouillon titrated to +1.5 per cent to phenolphthalein. The bee-larvae 
Fig. 3.— Bacillus orpkeus: Smear from a 4-day^old agar culture. X 1,480. Note the excentrically placed 
spores. 
agar was prepared by picking young bee larvae from the combs, crushing, 
straining through cheesecloth, adding three times the volume of water, 
and then, as in the case of beef bouillon, heating in a water bath at a 
temperature of 6o° to 65° C. for one hour. After filtering, peptone and 
sodium chlorid are added, and the reaction adjusted. To this, bee-larvae 
bouillon titrated to +1.5 per cent to phenolphthalein, 1.25 to 1,50 per 
cent of agar is added. 
AGE OF CULTURES AND METHOD OF INOCULATING 
The age of the cultures at inoculation varies considerably. The usual 
method of securing cultures for inoculation is to sow the whole number 
of slopes designed for one colony at one time. Usually 30 slopes are fed 
