Mar. ia, 1917 
Spore-Forming Bacteria of the Apiary 
4i 5 
hives, seasonal range, and various other data by separate years, together 
with totals of same for the whole five years' inoculations. 
Table I .—Results of inoculation experiments with organisms of the Bacillus vulgatus 
group « 
Number of 
Num- 
equivalent 
colonies 
fed cultures 
ber of 
strains 
re- 
Number of 
slopes of agar. 
on 
Total 
inocu- 
peated— 
i. e., 
used 
Num¬ 
ber of 
differ¬ 
ent 
colo¬ 
nies. 
Number of 
lations 
Seasonal 
Year. 
different 
equiva- 
on 
inoculation 
strains. 
lent to 
both 
range. 
Bee- 
larvae 
Ordi¬ 
nary 
colo¬ 
nies. 
bee- 
larvae 
and 
Bee- 
lar¬ 
vae 
Plain. 
To¬ 
tal. 
agar. 
agar. 
ordi¬ 
nary 
agar. 
agar. 
X9XX. 
6(2329,2094,2291, 
2442, B. vulga- 
tofB. A.I.,ba- 
^ 6 
*4 
10 
& 4 
4 
July 26-Sept. 16. 
168 
117 
285 
cillus from po¬ 
tato). 
1912. 
4(2904,2905,3309, 
c a 
4 
6 
ca 
6 
June 28-Sept. 25. 
60 
118 
178 
and *225). 
6(3932,3969, 4 I02 > 
4107, 4019, and 
4016). 
0 
6 
6 
(<9 
e 2 
June 39-Sept. 27. 
174 
174 
8(4277,4288,4292, 
4323,4381,4429, 
0 
8 
8 
(d> 
f 4 
June 12—Oct. 5... 
218 
218 
4438, and 5356). 
Of) 
191S. 
4(4473,4709,4727, 
and 4823). 
4 
0 
4 
2 
Aug. 2-Sept. 21.. 
107 
107 
Total. 
28. 
12 
22 
34 
6 
18 
Junei2-Oct. 5... 
33 S 
627 
962 
a Culture numbers refer to the laboratory numbers of samples of brood from which culture was obtained. 
& 2329, 2094, 2291, and 2442. 
c 2904 and 2905. 
d Used only on ordinary agar. 
« Three used also last year. 
/ One of these fed three cultures: 4288, 4323, and 4438. 
9 Used only on bee-larvae agar. 
In a consideration of the number of colonies inoculated it will be seen 
that a different colony was not always used for each strain. This was not 
necessary or even desirable, since, if one strain shows no pathogenic 
effects upon feeding to a colony, the same colony may be used over and 
over again, as often as convenient, for the inoculation of other strains. 
There were used, for the five years, 18 different colonies, which, with the 
repeated inoculations of some colonies, brings the equivalent number of 
colonies up to 34. Twenty-eight different strains of organisms represen¬ 
tative of the B . vulgatus group were inoculated. Twelve of these cultures 
were grown on bee-larvae agar and 16 on ordinary agar, as prepared from 
beef bouillon. Six of the 28 strains were grown upon both ordinary agar 
and bee-larvae agar. The total number of slopes inoculated was 962, of 
which 335 were on bee-larvae agar and 627 on ordinary agar. The sea¬ 
sonal range of the inoculation extends from June 12 to October 5, which 
is the seasonal range of the inoculations for the year 1914, as can be seen 
from Table I. 
78366 °— 17——2 
