Mar. 19, 1917 Transformation of Pseudoglobulin into Euglobulin 453 
Table I .—Weights of euglobulin obtained from 50-c. c . portions of serum 
Serum. 
Without 
heat. 
With heat. 
Increase in 
weight. 
Anthrax 48 . 
Gm. 
0. 774 
• 547 
. 406 
•435 
•243 
Gm. 
I. 201 
•793 
.719 
a . 642 
•304 
Gm. 
O.427 
. 246 
•313 
. 207 
. 061 
Anthrax 96. 
Diphtheria 1 . 
Do. 
Tetanus 1 .... . . 
a Result low, probably due to incomplete flocculation. 
The figures in Table I show unmistakably that more euglobulin pre¬ 
cipitate was obtained from the heated serum than from the unheated. 
Obviously, it was desirable to ascertain definitely where the excess of 
euglobulin came from, and to check the correctness of these single results. 
Concordant duplicates do not prove correctness of results in these analy¬ 
ses; they do prove uniformity of technic and uniformity of error. 
In the supernatant liquid (filtrate) poured off the euglobulin precipi¬ 
tates, the following determinations were made: Total coagulable protein 
(pseudoglobulin plus albumin), pseudoglobulin, and albumin. The 
method used was as follows: 
Total coagulable protein. —Duplicate portions of the filtrate of 20 
c. c. each, containing 9.35 c. c. of serum, were transferred to 400-c. c. 
beakers; about 300 c. c. of water were added and the mixture brought 
to a boil. The coagulum, consisting of pseudoglobulin and albumin, 
flocculated easily. This was filtered on weighed papers as usual, dried, 
and weighed. The results are tabulated as item C in Table II. 
Pseudoglobulin. —A single portion of 25 c. c. of the filtrate, containing 
11.68 c. c. of serum, was used. To this, 10 c. c. of saturated ammonium- 
sulphate solution were added, resulting in 50 per cent saturation. This 
was done in a 100-c. c. centrifuge tube and the mixture centrifuged for 
20 to 25 minutes at 2,500 revolutions per minute. The pseudoglobulin 
packed firmly in the bottom of the tube. The supernatant liquid was 
poured off and its volume noted. This is the solution used for albumin 
determination. The precipitated pseudoglobulin is dissolved in water, 
transferred to a 400-c. c. beaker, heated, coagulated, filtered, dried, and 
weighed as usual. The results are tabulated as item D in Table II. 
Albumin. —Of the above fluid from pseudoglobulin precipitation 25 
c. c., containing 8.34 c. c. of serum, was diluted and heated, and the 
coagulated albumin filtered, dried, and weighed as usual. The results 
are tabulated as item E in Table II. 
The figures for euglobulin in Table II, item B, are the same as those 
in Table I divided by 5. No corrections were made for the volume of 
the precipitate in any case. The main objects of obtaining these data 
were, as stated before, to ascertain the source of the excess of euglobulin 
78367°—17 - 2 
