454 
Journal of Agricultural Research 
Vol. VIII, No. is 
in the heated precipitates, and to determine the accuracy of the method 
of analysis. Theoretically, A should equal the sum of B and C, and C 
should equal the sum of D and E. Although this equality is absent, 
the differences appear to be consistent, and indicate a uniformity of error 
due, possibly, to the absence of corrections for volume of precipitate, 
solubility of the precipitate in the wash water, occluded ammonium 
sulphate, etc. These errors, however, do not invalidate a comparison of 
the data in one column with those of the same serum in the next. 
Table; II .—Analyses of heated and unheated serums . 
[All weights calculated to grams in io c. c. of serum.] 
Anthrax 48. 
Anthrax 96. 
Tetanus 1. 
Diphtheria 1. 
Serum. 
Un¬ 
heat¬ 
ed. 
Heat¬ 
ed. 
Un¬ 
heat¬ 
ed. 
Heat¬ 
ed. 
Un¬ 
heat¬ 
ed. 
Heat¬ 
ed. 
Un¬ 
heat¬ 
ed. 
Heat¬ 
ed. 
Un¬ 
heat¬ 
ed. 
Heat¬ 
ed. 
Gm. 
Gm. 
Gm. 
Gm - 
Gm. 
Gm. 
Gm. 
Gm. 
Gm. 
Gm. 
A. Total coagulable protein_ 
Do. 
0.674 
.618 
0.674 
.618 
0.789 
.788 
.109 
0.789 
.788 
• i 59 
0.558 
0.558 
0.665 
0.665 
0.665 
0.665 
B. Euglobulin. 
C. Pseudoglobulin and albu¬ 
•155 
. 240 
.049 
.061 
.081 
.144 
.087 
0 .128 
min estimated together... 
•437 
•378 
.668 
.625 
•447 
■ 451 
•534 
.487 
•523 
• 484 
D. Pseudoglobulin. 
.290 
. 211 
• 568 
•535 
•385 
•378 
•475 
•397 
•479 
•438 
E. Albumin. 
.191 
.191 
•133 
• 135 
• 103 
.103 
.090 
.09S 
. 087 
.097 
Pseudoglobulin converted 
into euglobulin. per cent.. 
27 
6 
0. 
2 
16 
9 
a Result low; see footnote to Table I. 
DISCUSSION OF RESULTS 
It will be noticed that the figures for albumin are practically identical 
in the heated and unheated serums. The figures for pseudoglobulin are 
consistently lower in the heated serums than in the unheated, which is 
an almost necessary consequence of the transformation of part of the 
pseudoglobulin into some protein having several of the precipitation 
characteristics of euglobulin. What is most important for the present 
investigation is that the loss of pseudoglobulin in the heated serums 
corresponds almost quantitatively with the gain in euglobulin in the 
same serums. 
Obviously the figures for euglobulin, pseudoglobulin, and albumin are 
interdependent, and an error in one may cause a corresponding error 
in another. The figures for total coagulable protein, however, are inde¬ 
pendent of the others, and an error in them has no direct influence on 
the figures for the others. The two figures for total coagulable protein 
in the anthrax serums were obtained January n, 1916 (upper figures), 
and May 9, 1916 (lower figures). The comparatively large difference 
between the two consecutive determinations in anthrax 48 may perhaps 
be due to the action of serum protease, which continued to digest the 
serum proteins. Chloroform does not prevent this action when the 
protease is active. The figures obtained were 0.676, 0.672, and 0.616 
