ALVAREZ-CAMPOS ET AL.: NEW SPECIES OF MYRIANIDA FROM LUZON 
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consensus sequences with Geneious 6.1.6 (Druminond et al. 2009). In order to establish the phylo¬ 
genetic position of M. pidadilaw sp. nov. within the genus, we used nuclear (18S) and mitochondr¬ 
ial (16S and COI) GenBank sequences of 18 Myrkmida species provided by Nygren et al. (2010). 
Speeies of Proceraea, Epigamia, Imajimaea, and Vichowia were used as outgroups (Table 1). 
The mitochondrial data sets were analysed both separately and combined with nuclear data. 
RAxML 1.3 (Stamatakis 2006) was used to perform the Maximun likelihood analysis. Bootstrap 
support values were estimated by 1000 replicates, implementing a thorough bootstrapping algo¬ 
rithm (Stamatakis et al. 2008). The Bayesian hiference analysis was performed in MrBayes 3.2.1 
(Ronquist and Huelsenbeck 2003). Four Markov chains were started from a random tree and run 
simultaneously for one million generations with trees sampled every 500 generations (samplefreq 
= 500); the initial 25 pereent of trees being discarded as bumin (buminfrac = 0.25). The model of 
Table 1 . Species included in phylogenetie analyses, collection localities, voucher and Gen- 
Bank aecession numbers. 
Locality 
Voucher 
COI 
16S 
18S 
Epigamia alexandri 
Kaldbak, Faroe Islands 
SMNH 45824 
GQ856183 
AF474262 
AF474308 
E. magna 
Friday Harbor, Washin- 
ton, USA 
SMNH 45825 
GQ856185 
AF474263 
AF474309 
Myrianida amtraliensis 
Kaikora, New Zealand 
GNM Polychaeta 13105 
GQ856187 
EF661834 
EF661838 
M. brachycephala 
Kristineberg, Sweden 
SMNH 45812 
GQ856188 
AF474250 
AF474296.1 
M. convoluta 
Santa Catalina, California, 
USA 
SMNH 45817 
GQ856189 
AF474257 
AF474303 
M. dentalia 
Friday Harbor, Washin- 
ton, U SA 
SMNH 45819 
GQ856190 
AF474301 
AF474301 
M. edwardsi 
Kristineberg, Sweden 
SMNH 45810 
GQ856191 
AF474248 
AF474294 
M. flava 
Friday Harbor, Washin- 
ton, USA 
SMNH 45818 
GQ856192 
AF474254 
AF474300 
M. gidholmi 
Carrie Bow Cay, Belize 
GNM Polychaeta 13103 
GQ856193 
EF661835 
EF661839 
M. hesperidium 
Ban 3 ails, France 
SMNH 45815 
GQ856194 
AF474259 
AF474305 
M. inermis 
La Jolla, California, USA 
SMNH 45821 
GQ856195 
AF474256 
AF474302 
M. langerhansi 
Banyiils, France 
SMNH 45813 
GQ856197 
AF474251 
AF474297 
M. longoprimicirrata 
Banyuls, France 
GNM 13214a, b 
GU722619 
GU722629 
GU722643 
M. pachycera 
Los Angeles, California, 
USA 
SMNH 45816 
GQ856198 
AF474258 
AF474304 
M. pentadentata 
La Jolla, California, USA 
SMNH 45820 
GQ856199 
AF474253 
AF474299 
M. pinnigera 
Cadiz, Spain 
SMNH 45823 
GQ856200 
AF474252 
AF474298 
M. puladilaw 
Batangas, Philippine Ids 
MNCN 16.01/14734 
KJ534506 
KJ534507 
— 
M. pwlifera 
Kristineberg, Sweden 
SMNH 45811 
GQ856201 
AF474249 
AF474295 
M. quindecimdentata 
Banyuls, France 
SMNH 45814 
GQ856202 
AF474260 
AF474306 
M. rubwpunctata 
FeiTol, Spain 
GNM Polychaeta 13106 
GQ856203 
EF661837 
EF661841 
Proceraea albocephala 
Porto Moniz, Madeira 
GNM Polychaeta 13194a, b 
GU722607 
GU722640 
GU722646 
P madeirensis 
Funchal, Madeira 
GNM Polychaeta 13208 
GU722617 
GU722627 
GU722645 
P pleijeli 
Cadiz, Spain 
SMNH 5947 
GQ856167 
AF474272 
AF474318 
Virchowia clavata 
Banyuls, France 
AN 
GQ856171 
AF474268 
AF474314 
