Baird—History of Phycornyces Nitens (Agardh) Kunze. 363 
quently observed in poorly nourished hyphae. Burgeff calls the 
deep-staining body chromatin, states that it is nucleole like, and 
that it is frequently located eccentrically in the nucleus. In well- 
nourished hyphae the chromatin body is much larger than in poorly 
nourished hyphae. His special study of the nuclei has been in the 
galls formed at the point of attack of the parasite. He points out 
that the nuclei in the sporangiophores contain a much larger 
chromatin body than do those in the vegetative branches; he 
attributes this to the presence of reserve food in the sporangio¬ 
phores. He states that the Mucor nuclei in the gall divide mitoti- 
cally, but gives no figures nor details of the process. He also de¬ 
scribes crystalloid bodies as arising from degenerating nuclei. 
Materials and Methods 
The material from which the preparations were made was grown 
in petri dishes on potato-glucose agar prepared with distilled water. 
Flemming’s medium fixing reagent was used almost exclusively, 
after a wide variety of reagents was tried out. The imbedded ma¬ 
terial was cut in sections 4/x-12/x thick and the preparations stained 
with Flemming’s triple stain. Heidenhain’s iron-alum-haematoxy- 
lin method of staining was also used, but in no case gave the 
minute differentiation obtained by the use of the triple-stain. 
For the germination of the zygospores of Phycomyces, zygospores 
that had remained on the original substratum, upon which they 
grew, in a dark room at an average temperature of 20 degrees C., 
germinated readily when transferred to a non-nutrient 2% agar 
medium. The zygospores germinated, forming a single germ- 
sporangium within four or five days after transferring. The cul¬ 
tures of germinating zygospores were grown in a light room, at 
room temperature. On account of interruption in the work, the 
study of nuclear behavior in the germ sporange and of the sex of 
the germ-sporangiospores has not been completed. 
Phycomyces Nitens 
In the swollen spores of Phycomyces about to germinate there 
are one or more vacuoles with several (4-13) nuclei imbedded 
within the cytoplasm. After the germ tube has been formed 
(fig. 1) the nuclei are distributed throughout the enlarged cell 
by the increase of vacuolar volume. The nuclei show no change 
in structure up to this period. So far as I have observed the 
