440 
E. A. DE SCHWEINITZ and F. L. KILBORNE. 
of Agriculture for 1891. The albumose was best obtained 
from the cultures, after the removal of the germs by means 
of a Pasteur filter, by precipitation with absolute alcohol, 
resolution in water and reprecipitation. 
In the July number, 1892, of the Archives de Medecine Ex- 
perimentale et d’Anatomie Pathologique, A. Babes describes 
the isolation in the same way of apparently the same substance- 
from glanders cultures. He claims with this to have been 
able to immunize guinea pigs against glanders. He has either 
failed to see or note our earlier experiments. 
During the present year we have, at the direction of Dr. 
D. E. Salmon, turned our attention to the use of mallein as a 
means of diagnosing glanders. 
In the experiments made by Preusse, Nocard,* Tietze,f 
EberJ and others, the mallein used was prepared, by scraping 
the surface growth from potato cultures, dissolving in water, 
sterilizing, by heating to ioo° C., filtering, and injecting upon 
the addition of an equal volume of a two per cent, solution of 
carbolic acid. 
Our mallein was prepared in the following way : Acid 
peptonized beef broth cultures containing five per cent, of 
glycerine were inoculated with the glanders bacillus, and thej 
cultures allowed to grow for two months at the .temperature 
of the room. At this time the growth, which had been very I 
abundant, had almost entirely ceased. The liquid was then 
heated for two hours from 8o° to ioo° C., after which it was 
filtered through a Pasteur tube to remove the germs. The 
resulting clear amber liquid, after being tested to prove the 
absence of germs and diluted with 50 per cent, of glycerine 
for better preservation, were used for injections. A purer 
lymph was obtained by the precipitation of the filtered cul¬ 
tures with absolute alcohol, and resolution in 50 per cent, gly¬ 
cerine. For practical purposes the crude lymph is equally as 
good. The greatest care and precautions have to be observed 
*Nocarcl, Recueil de Med. Yeter. 1892, No. 8, p. 209. 
fEber, Recueil de Med. Yeter. 1892, No. 8, p. 86. 
JTietze, Berl. Tkierarz. Woch. 1892, No. 8, p. 86. 
