THE USE OF MALLEIN. 
441 
all of the work that the cultures are pure, of the proper age, 
id that the lymph is perfectly sterile and can be so preserved, 
are must also be observed in making the injection to avoid 
ie danger of contamination or infection by means of the 
cringe. 
The value of the mallein prepared as described, as a di- 
piostic agent for glanders in horses, has been extensively 
ied at the experiment station of the Bureau of Animal In- 
ustry; and also by Doctors Francis of Texas. Dinwiddie of 
rkansas, and Casewell of Chicago. Lymph was also sent 
• several other experiment stations and veterinarains, but 
iey have, as yet, failed to report. The results of these tests 
e recorded in the accompanying tables. 
As sent out for use the lymph is diluted with an equal 
}lume of glycerine, so that it will keep better. 
The amounts of mallein named in the charts refer to the 
idiluted solution. Of the diluted solution just double the 
lantity was given. 
At first we made injections with icc. of the mallein (2cc. 
the 50 per cent, solution), but finding this to be apparently 
o large a dose, as it sometimes gave a decided febrile reac- 
on in healthy horses, we have decreased the quantity to 
15CC. of the mallein, or icc. of the 50 per cent, solution. But, 
dging from the results being obtained from some experi- 
ents now in progress, we may find it desirable to return to 
e larger dose, and depend on a second injection of those 
hrses which gave a doubtful reaction for a positive diag- 
>sis. 
In several instances the same horses have been repeatedly 
jected. To indicate this in the charts a figure in brackets 
placed at the head of the column showing the tempera- 
res, which figure indicates the number of times the horse 
is been injected. 
With the exception of a few of the earlier injections which 
ere made over the shoulder, we have made the injection 
:ar the middle of one side of the neck, where any local 
celling can be readily detected. In our experiments the 
cal reaction, or swelling, in the glandered horse has been 
