THE USE OF MALLEIN. 
445 
id appear, it developed in two to six hours, and had disap- 
sared again by the next day. 
Horse No. 7 received a daily injection of 1 c.c. of the 
allein from May n to June 23, inclusive. During this time 
tere was no elevation of temperature, which varied from 
).5 q F. to 101.6 0 F. But after each injection a local swelling 
Dpeared, varying in extent from six to ten inches across, and 
isappearing at the end of about the third day. During the 
st week of the injections the swellings were less marked, but 
ill invariably followed each injection, otherwise no effect 
as noted from the continued treatment. 
On horse No. 21, the farcy buds had entirely healed within 
week after the first injection, and on No. 20 the extensive 
rcy swellings had all healed, July 15th. Except a slight 
scharge from the left ear of No. 20, these two animals have 
mtinued apparently entirely well, and have improved in 
bneral health since the above dates. However, the last in- 
ctions of the mallein, August 27th and 30th, caused a decided 
se in temperature and a marked characteristic swelling at 
e point of injection, which would indicate that the horses 
re not free from disease. 
Table V shows the effect upon horses of the albumose ex¬ 
acted from the cultures. In the first of the experiments, 
■ie amount used, 0.005 grammes, was evidently entirely too 
Tge, causing almost as much reaction in the healthy as in 
\e glandered horses. In the second and third experiments 
e dose was reduced to 0.002 grammes per horse, but this 
as also slightly too large. This table serves to show the 
owerful effect of the poison secreted by the glanders bacil- 
s. A dose of only 0.001 grammes of the albumose would 
"obably be sufficient to give a decided reaction. 
In conclusion, the tests made show that mallein is of great 
ilue in the diagnosis of glanders in horses. The injection 
1 0.5 or 1 c.c. of the mallein may cause a slight rise of tem- 
Drature in a healthy horse, but it will rarely give a reaction 
oon a second injection ; and there will not occur on the 
ialthy horse any marked swelling at the seat of the injec- 
On. On the glandered horse, the first injection always gives 
marked febrile reaction (except as noted) and a large swell- 
