446 E. A. de SCHWEINITZ and F. L. KILBORNE. 
ing at the seat of injection. Subsequent injections also giv 
a similar reaction, although frequently less marked. Health 
horses readily acquire an immunity to large doses of the ma 
lein, not so acquired by glandered horses. The active prii 
ciple in mallein is an albumose which can be precipitate 
from the cultures by means of alcohol or ammonium sulphat< 
Further experiments may prove that a preventive inje< 
tion or treatment of glanders in horses is possible. The d 
rections, which were sent out to serve as a guide in testin 
the value of the mallein, may be conveniently given here fo 
future reference. 
Make the test, if possible, with a healthy horse, as well a 
with one or two more affected with glanders. Take the terr 
perature of all these animals three times a day for one or tw 
days before making the injection. On the day of making th 
injection, take the temperature every two hours from earl 
in the morning until late in the evening. Use for each hors, 
one cubic centimeter of the solution as sent you, and mak 
the injection beneath the skin of the shoulder. Be carefr 
and thoroughly sterilize the syringe after injecting eac 
horse, or better, use separate syringes for healthy and sus 
pected animals. If the same syringe must be used, inject th 
healthy animals first, and thoroughly sterilize the syringji 
after each of the other injections. Sterilize the thermometer 
in carbolic acid after taking the temperature of each horse 
The temperature will begin to rise, as a rule, from three ti; 
four hours after the injection, and reach its maximum eight 
to ten hours after injection. On the two days succeeding th I 
injection, take the temperature three times a day. Note th I 
general condition of the animal both before and after the ir 
jection. After four or five days, the injection should be re 
peated. Keep the solution in a sealed bottle, and in a coc 
place. As sent from here it is free from germs. 
So far as we have experimented, the mallein has caused ;« 
rise in the temperature of all horses affected with glanders' 
but it is possible that it also, in rare instances, causes a rise h 
the temperature of horses that are not affected with this die 
ease. We deem it important that this latter point should b 
definitely determined by a large number of observations. 
