A STUDY OF SEPTIC GERMS. 
607 
September 27, inoculated an old buck rabbit subcutaneous¬ 
ly with the streptococcus pyogenes obtained from the colt, 
using the second transfer. Transfers were made May 10, 
1892 and June 13, 1892. October 10, the rabbit remained 
healthy, no effect having been produced. 
Some experiments were made with the staphylococcus py¬ 
ogenes varieties aureus and citreus to determine the length of 
time the germs retained their pathogenic properties. 
August 1, inoculated a mouse with staphylococcus pyog¬ 
enes, var. citreus, using the fourth culture. Cultures were 
made October 31, 1891 ; February 10, 1892 ; June 2, 1892 and 
July 29, 1892. It produced no^effect, the mouse remaining 
healthy. 
September 9, inoculated intraveinously an old buck rab¬ 
bit with the staphylococcus pyogenes, var. citreus, using the 
fourth transfer. Transfers were made October 21, 1891 ; 
February 10, 1892 ; June 2, 1892; July 29, 1892. It produced 
no effect. The original culture had been very pathogenic. 
September 18, inoculated a spotted rabbit with staphylo¬ 
coccus pyogenes, var. aureus, using the last transfer. Trans¬ 
fers were made October 26, 1891 ; February 10,1892 and July 
29, 1892. It produced no effect. 
The cultures being nine months old August 1st and non- 
pathogenic, proves that the germs staphylococcus pyogenes 
aureus and citreus lose their pathogenic properties within 
that time. 
Further experiments with the staphylococcus pyogenes 
aureus were as follows:—September 20, inoculated a small 
rabbit subcutaneously, using the last culture. Cultures were 
made August 22, 1892 and September 12, 1892. It resulted 
in a small abscess at the point of inoculation, followed by an¬ 
other at the base of the left ear. Otherwise the rabbit re¬ 
mained healthy October 8, 1892. 
October 7, inoculated a white rabbit with staphylococcus 
pyogenes, var. aureus, using the last transfer. Transfers 
were made August 8, 1892 and September 12, 1892. Death 
occurred in thirty-six hours from general septic infection. 
There were no local sy my toms. The germ was obtained pure. 
