616 
C. B. ADAMS. 
that they are correct, and convey a correct idea of the vir. 
tues of the drug-. 
A few slight irregularities may be observed in the results. 
The reader will have to remember the many difficulties at 
tending the making of cultures. Perhaps in one or two cases 
oui means of contention against those obstacles were not suf¬ 
ficiently observed, or the germs in one solution may have be¬ 
come aggiegated into masses to a more or less degree ; as we 
are dealing with a mechanical suspension, this is very likely 
to occur. I have in a great measure followed the method 
used by Prof. John Abbot, of Hopkins University. After forty- 
eight hours giowth of the germ in bouillion culture media, 
one cc. of the media containing the germ was placed in 2c 
cc. of the lysol solution. With a measured dropping pipette 
one drop of the solution containing the germ was transferred i 
to io cc. stei ilized water, for the purpose of so diluting the! 
disinfectant as to insure no farther action, one drop of this 
was then tiansfeired to io cc. agar agar, at a temperature of 
4 °° C., and plated according to the Koch method. 
The inoculations were made from the solution, after various ^ 
lengths of time, as seen by the experiments. jj 
Series I. 
Exp. i : i per cent, solution, i cc. aureus culture, grown! 
48 hours in bouillion, was placed in solution (20 cc.) and the 
plates made as follows : 
The one taken immediately, 8; in 5 minutes, 0; in 10 min¬ 
utes, o; control plate, countless. Counted after 48 hours’ 
growth. 
2. £ pei cent, solution. 5 minutes, ©; 10 minutes, o; 20 
minutes, o ; control, 526. Counted after J2 hours. 
3. -g- pei cent, solution. 5 minutes, 1; 10 minutes, o; 15 
minutes, o; control, 1,652. 72 hours’ growth. 
Series II. 
1. 1 percent, solution. 1 cc. aureus culture grown 48 hours 
in bouillion, placed in solution. 5 minutes, 3; 10 minutes, 0; 
15 minutes, o; control, 38. 48 hours’ growth. 
