C. B. ADAMS. 
618 
a spore-forming organism, for the purpose of finding out the 
resistance offered by the spores on the various solutions of 
lysol. After 72 hours’ growth of the germ in bouillion, it 
was filtered through glass wool. The operation from here 
on is exactly the same as those with the aureus. 
Exp. 1. 1 cc. bacillus subtilis culture, grown 72 hours in 
bouillion placed in solution. 1 per cent, solution. Immediatej 
5 ; 5 minutes, o ; 10 minutes, o; control, 497. 72 hours’ growth. 
2. 1 per cent, solution. 5 minutes, 5; 10 minutes, 2; 15 
minutes, o; control, not good. 72 hours’ growth. 
It is probable that the inoculation for the control plate! 
was taken from the wrong water tube, as the germ was passed 
through 10 cc. water before putting it on the agar plate. 
3. 2 per cent, solution. 1 cc. sub. culture grown 48 hours 
in bouillion was placed in solution. 5 minutes, o ; 10 minutes 1 
o; 15 minutes, o; control, 13. 72 hours’growth. 
4. 2 per cent, solution. 5 minutes, o; 10 minutes, o; 15 
minutes, o ; control, 497. 72 hours’ growth. 
The experiments show that a 1 per cent, solution is not oil 
sufficient strength to overcome, in every case, the resistance: 
of the spore, but a 2 per cent, solution’s action was positive 
in every case. 
CONCLUSION. 
After summing up the results of our investigations, whai 
are the conclusions. Our sphere of investigation embrace! 
only disinfectant properties of lysol. A glance, however, a 
the physiological properties, and a few of its virtues, in gen 
eral, will strengthen what may be said of it as a disinfectant 
From experiments we are convinced of its powerful germ 
icidal action. It is deserving of a high rank. 
1st. Because of its power in weaker solutions, as compared 
with carbolic acid. 
2d. Because it is but slightly, if at all, poisonous to th<jj 
system, as shown by Dr. Michelson, who flooded the abdomi) 
nal cavity with a per cent, solution, and did not observe 
even the slightest symptoms of poisoning, and also Drs. Gerl 
lach and Sugg, who experimented separately, and arrived a 
