322 
EXTRACTS FROM FOREIGN PERIODICALS. 
however, in the majority of cases where such old nodules 
have been used in confirming the disease, the result has been 
unsatisfactory, we can attach no importance to this instance, 
which rather proves, than otherwise, the rule. 
Again, in horses Nos. XXXI. and XXXV., the variation 
after the administration of the virus was not as expected, 
though the increase actually happened, yet it was within the 
limit of normal variation. All other horses used in these ex¬ 
periments gave positive results. 
The interim between the injection and the appearance of 
the rise in the temperature, in no two cases was precisely the 
same. In one case of acute cuticular glanders the afflicted 
horse manifested the change within four hours after the ap¬ 
plication of the glanders lymph, while in another patient 
suffering pulmonary glanders the change was first visible 
twenty hours later. On an average, however,^we may expect 
the augmentation nine and one-half hours after the introduc¬ 
tion of malleine. 
This discrepancy may hatfe been due to the variable quan¬ 
tity of the active principle present in the lymph injected. 
The duration of the elevation we determined to be more con¬ 
stant, reaching its maximum in from six to four hours and 
thereafter gradually declining. The operation of malleine is 
analogous to that of taberculin, in that the structure acted 
upon is immediately adjacent to the nodules, or it is the 
nodule itself. The red zone surrounding the pulmonary and 
splenic nodules, as shown by post mortem, is due to the in¬ 
flammatory effect of the malleine. 
In one case, No. VII., the ulcerous wounds upon the 
pituitary mucous membrane diminished in size, but in other 
cases where no visible glanderous symptom was evident, the 
same appeared in a few days. 
In closing our report we desire to offer a few suggestions 
upon the practical application of malleine injections. The 
dose is dependent upon the degree of concentration of the 
lymph ; on account of the changeable composition of the 
lymph it seems advisable to first try small quantities—3ss— 
upon a known glanderous horse, afterward regulating the 
dose accordingly. 
