American Veterinary Review, 
MARCH, 1893 . 
ORIGINAL ARTICLES. 
EPITOME UPON MALLEINE INOCULATIONS. 
Translated by Richard Middleton, D.Y.S. 
The discovery of Robert Koch—that a special extract from a 
pure culture of tubercle bacillus possessed a specific property 
when injected into the organism of consumptive animals—has 
been the incentive to other bacteriological investigators to apply 
analagous extracts to the eradication of other infectious diseases. 
In veterinary medicine, the effete product and protein body 
of the glanders bacillus first came under consideration; the great 
similarity between the latter disease and tuberculosis readily 
leading to the opinion that in this affection such a specific 
action might be hoped for. The successful application of this 
conviction was reserved for Kalning 1 , of the veterinary institute at 
Dorpat, Russia. He derived a lymph by consecutive cultures of 
the malleus bacillus, which induced noticeable symptoms in 
afflicted animals, but caused no alteration in healthy beings. 
This virus, as it may be conveniently designated, was pre¬ 
pared by Kalning in the following manner: Take of the pure 
culture of glanders bacillus 5 g., add thereto 20 cubic centime¬ 
ters of sterilized distilled water, placing the mixture in the ther¬ 
mostat regulated to 248° F. for twenty minutes. Within the 
(l)Zur Diagnose des Rotzes. (Archiv. fur Veterinar wissen-schaften Bd. I., 
April-May, 1891, Petersburg). 
