EPITOME UPON MALLEINE INOCULATIONS. 
669 
hours subsequent to the first, a second inoculation was under¬ 
taken, with an increase in the dose to 3.0-5.0 ccm. (4.0-7.0 g. 
malleine). The operation of the same was highly interesting ; 
five of the horses offered variations in the temperature, which fif¬ 
teen hours after the first and eight hours after the second appli¬ 
cation had reached its zenith. The deviation occurred between 
2.7 0 and 3-9° F.; the sixth horse, however, a two-weeks-old foal, 
manifested an increase, although exceedingly slight, amounting 
to 0.9 0 F., which after the last injection became normal. 
In addition, each of the first five horses treated exhibited a 
doughy swelling about six inches in diameter which was painful 
upon pressure, involving the subcutaneous connective tissue. On 
the following day the subjects appeared apathetic and drowsy. 
The sixth, the foal, showed none of these characteristics. On the 
third day the post-mortem confirmed the suspicions regarding the 
five adult animals ; in short, the malleine had reacted as a de¬ 
veloper of otherwise latent dispositions of the glanderous disease. 
Preusse next obtained a horse sound in every respect. To 
this animal he gave the malleine in large doses ; in forty-eight 
hours no abnormal variation of the temperature was discernible, 
notwithstanding the fact that the same received 1.8 ccm. of the 
pure lpmph. The autopsy confirmed the action of the virus. 
Opposed to the foregoing it is to be recorded that Preusse inject¬ 
ed 0.5 g. of malleine in an intra-vitam glanderous patient, with 
the result that in nine to eleven hours the temperature advanced 
2.7 0 F., which, upon repeating the dose, advanced .72° F. fur¬ 
ther. The “ Preusse ” lymph has been tried by numerous others, 
so that the record of the same is most complete. With this lymph 
Heyne-Posen 1 inoculated twenty-three head, Schillingoppeln 2 nine, 
Peters and Felisch 3 ten and Dickerhoff 4 and Lothes seventy. Of 
these one hundred and twelve horses the greater number gave no 
(1) Versuche mit Rotzlymphes (Mallein) bei Pferden. (B. T. W., 1891). 
(2) Experimenteller Beitrag zur Verwerthung des Malleins fur Diagnose des 
Rotzkrankheit. (Ebenda, 1891). 
(3) Beitrag zu den Impforosuchen mit Preussescher Rotzlymphe (Mallein) 
bei Pferden. (Berliner Thierarz Wochenschr., 1891). 
(4) Beitrag zur Beurtheilung des Mallein. (Ebenda, 1891, 1892). 
