670 
R. MIDDLETON. 
infallible sign that the dreaded affection had located within their 
organism. 
In sixty-six of these cases, a reaction followed nine to ten 
hours after the injection, on an average, in doses of 0.5 g. mal- 
lein diluted with 4.5 g. of one per cent, carbolic solution; in the 
remaining forty-six no appreciable deviation from the normal. 
An autopsy upon the former revealed sixty three possessing the 
internal lesions of the affection; in two (Peters and Felisch, 
Dieckerhoff and Lothes) no pathological change could be noted, 
despite the most painstaking means. In one (D and L), a for¬ 
mation in the lungs which evidenced in its macroscopical charac¬ 
ter the glanders nodule, but which when applied to guinea-pigs 
gave negative results. 
The post mortem upon the forty-six animals showing no 
symptoms, seconded the intra-vitam diagnosis. 
In addition to the pyrexia, the afflicted subjects manifested 
great weakness, immobility of the sensorium, accelerated pulse 
and respiration with absence of appetite. Structural changes at 
the point of introduction were not uniform, the commonest lesion 
being an inflammation of an ephemeral nature which disappeared 
in the next few days. 
Most interesting to note, is the fact that in all of those cases 
decidedly glanderous the circumscribing pulmonary and splenic 
tissue arranged itself in a zone of inflammatory quality, with the 
nodule as the central point. 
Dieckerhoff and Lothes instituted further experiments with 
the object in view of determining the effect of the lymph when 
used upon horses afflicted with pleuro-pneumonia, rheno-adenitis, 
chronic inflammation of guttural pouches, sarcoma of same, etc. 
None of the animals in the trials were affected by the malleine ; 
and no horse, free from malleus, exhibited fever reaction even 
when receiving three doses of the malleine. 
Pearson obtained his malleine by adding salt and glycerine 
to a bouillon which had flowed over virulent potato cultures ; 
subjecting the same, through fourteen days, to a heat of 97 0 F. in 
the thermostat, and again later, for several hours to 176° F. 
This thickened fluid, after being filtered through baked clay, be- 
