EPITOME UPON MALLEINE INOCULATIONS. 
671 
came perfectly transparent; the same was sterilized by placing 
for twenty minutes, each day for three days, in a stream of flow¬ 
ing steam. When 0.25 to 2.0 ccm. of this fluid was subcutane¬ 
ously injected into glanderous guinea-pigs, the body heat ad¬ 
vanced in a decided degree, and redness, with swelling adjacent 
to the inoculation area were observed. In healthy animals the 
repeated injections produced only a local reaction and insignifi¬ 
cant fever. 
Since all fluid extracts of bacteria cultures are irregular in 
their consistency, and especially so regarding the active principle, 
Foth 1 and Gutzeit 2 have had in view the production of this active 
substance or essence in a more solidified and substantial form. 
Foth for this purpose procured three various kinds of malleine, 
which he distinguished as I, II and III. 
Malleine I was prepared as Preusse describes, from potato 
cultures; malleine II from pepton-agar cultures containing gly¬ 
cerine; malleine III from the glycerine-buillon cultures. By ad¬ 
hering to Koch’s method of procuring pure tuberculin, he pro¬ 
ceeded with his effort to secure pure malleine. From malleine 
III there precipitated a yellowish flaky coagulum on the addition 
of absolute alcohol; by washing several times upon the filter with 
absolute spirits the same became grayish and unctuous. In the 
exsiccator, over chloride of lime or sulphuric acid, this product 
dries to a crumbling, white and spongy mass, which is easily 
pulverized and soluble in water, being about one-half per cent, of 
the original fluid. Similar results are secured by using malleine 
I and II, though the per centum is materially smaller. 
This precipitate dried over sulphuric acid has been used by 
Foth upon field mice and guinea-pigs. Three field mice, which 
had been inoculated with glanders twenty-four hours previous, 
received of this powder 2-5 milligrams and succumbed twelve 
hours after; ‘‘control” mice inoculated at the same time and 
taking none of the powder, died four days after. An exquisite 
(1) Untersuchung ueber die wirksamen Bestandtheile des Malleins. fZeitsch. 
fur Veterinarblunde, 1892, No. 3). 
(2) Ueber Mallein. (Ebenda 1892, No. 4 und 5) 
