672 
R. MIDDLETON. 
subject of glanders, a guinea-pig, gave no response upon the ad¬ 
ministration of 0.01 gramme, but succumbed twenty-four hours 
after the injection of 0.03 grammes of the powder. Foth believes 
himself justified in assuming his product to contain at least some 
of the active principle. 
Gutzeit has likewise succeeded in attaining similar results. 
He obtained his malleine from a bouillon culture—matfe from 
beef and horse meat with the addition of salt and peptone— 
maintained fourteen days at 98° F. and over. He now concen¬ 
trated this gradually over the water bath, filtering it, and at last 
secured one-quarter of the original quantity ; this was of a clear, 
wine-red color and neutral in reaction. 
With this he inoculated ten horses, of which eight manifested 
an advance of i.9°-5.2° F. in temperature, which lasted eight to 
ten hours; the other subjects remained normal. 
The former exhibited glanderous lesions upon post mortem ; 
the latter proved to be in a healthy condition. Gutzeit, in ex¬ 
periments upon the relative virulence of antiquated malleine, 
communicates that a preparation a few weeks old possessed 
activity, but in a lesser degree. 
Finally, Gutzeit made investigations calculated to determine 
the chemical constitution of his product. By the addition of 
absolute alcohol, or an alcoholic solution of mercury chloride, he 
was again successful in securing the active agent in the form of a 
precipitate. He recovered the same material from the fluids of 
the glanderous cadaver (guinea-pigs and horses) but not from 
bouillon. Doses of 0.01 gramme and o. 15 grammes of this dried 
alcoholic precipitate induced 2.5 0 and 3.4 0 F. of pyrexia in all 
inoculated guinea-pigs and horses. 
Gutzeit further eliminated from the alcholic precipitate an 
effective body whose watery solution produced fever in glander¬ 
ous guinea-pigs. 
In the military veterinary colleges in Berlin, malleine prepared 
according to Gutzeit—alcoholic precipitate dissolved in water— 
was used by Engelen and Willach 1 in seven cases, of which six 
(1) Malleinempfungen auf Grube Heintz-Dechen. (Zeitschrift fur Veterin- 
ank 1892, No. 6). 
