EPITOME UPON MALLEINE INOCULATIONS. 
673 
responded by an increase of 3.0 to 4.6° F.; the single remaining 
animal gave no sign of fever. Post mortem confirmed the diag¬ 
nosis as evidenced through the malleine. 
The rectal temperature in these six cases continued long at 
its abnormal height, assuming very slowly and irregularly its phy¬ 
siological standard, without disturbing the general condition. 
Exceedingly valuable are the instructions and tests of Nocard, 
which will here be exhaustively related. The investigator, in a 
correspondence to the Recneil de Medecine Veterinaire, in April, 
1892, defines the application and operation of malleine as pre¬ 
pared by Roux at the Pasteur Institute. This product, under 
certain limitations, was able to discern glanders when all other 
diagnostic agencies failed. Nocard emphasizes the importance 
of always using the same malleine, i.e., a product upon whose in¬ 
fallibility one has informed himself; such a material is to be pro¬ 
cured in the following manner : 
Through numerous passages it is possible to so augment the 
virulence of the glanders bacillus that rabbits which usually re¬ 
quire months, and white mice which possess, practically, immun¬ 
ity, succumb within thirty hours from the intensity of its propa¬ 
gation and ravages. The bacillus is sown in peptonized glycerine- 
bouillon (3 same as tubercle bacillus) and maintained for one 
month at 95 0 F.; after the expiration of this time the same is 
sterilized by subjecting to a torridity of 230° F. Now filter 
through paper, at a low temperature, in a vacuum in the presence 
of sulphuric acid, reduce its volume to one-tenth the original. 
By this procedure the fabricator is in possession of a syrupy 
fluid of a peculiar odor and dark brown color, which maintains 
its freshness for a long period in the glare of the sun. For pur¬ 
poses of application, a mixture of 1.10 carbolic solution (5.1000 
water) is used. This is uniform in its effects. 
When subcutaneously injected in healthy horses, in doses of 
y 2 -1 cubic centimeter, it causes a local cedematous swelling, hot 
and painful in character, which never suppurates and which sub¬ 
sides in one to two days. Contemporaneously there appears a 
febrile reaction lasting twelve to fifteen hours, and amounting to 
an advance of 2.7 0 3.6° F.; patient dull, inactive, trembling and 
without appetite. 
