676 
E. MIDDLETON. 
ive disease which depends upon the state in which the virus is 
incorporated in the blood. 
The favorable issue of these experiments, and the importance 
of the subject, induced the Bureau of Contagious Diseases con¬ 
nected with the Royal College at Munich to formulate similar 
trials. Assistant Hoflich prepared a lymph as instructed by the 
foregoing investigators. 
A peptonized bouillon made from horse meat, and to which 
glycerine had been added, was fructified with the glanders bacil¬ 
lus from potato cultures of the first generation. The same was 
placed in a breeding-oven thermostat, and maintained at 98.6° F. 
for approximately three weeks. The preparation, which had be¬ 
come opaque, thick and yellowish gray in color from the propaga¬ 
tion of the bacillus, was now tested relative to its containing the 
microbe. Sterilization was accomplished by placing in escaping 
steam. After percolating through ordinary filter paper and add¬ 
ing a 0.5 percent, carbolic solution, Hoflich obtained perfectly 
clear reddish brown liquid of singular odor. 
Six guinea-pigs, of which four had been artificially inoculated 
three weeks previous, were given injections of J^ccm., to which 
the same quantity of a 1 per cent, carbolic solution had been 
added. After five hours the four affected animals, which ex¬ 
hibited tumefaction of the inguinal glands, experienced a rise 
in temperature which reached its zenith five hours later. 
The table explains in detail the variations of temperature: 
INOCULATION ON JUNE 23, 1892, 8 O’CLOCK A. M., 0 . 5 g. 
MALLEINE. 
8 A.M. 
9 A.M. 
IO A.M 
II A.M 
12 A.M 
I P.M. 
2 P.M. 
3 P.M. 
4 P.M. 
5 P.M. 
6 P.M. 
7 P.M. 
8 P.M. 
Remar. 
Guinea-pig 
I 
37-2C- 
37-5 
37-° 
38.5 
38.8 
39-i 
39-o 
39-5 
39-7 
40.0 
40.2 
39 - 6 
39-0 
Gland* 1. 
U 
II 
38.4c. 
38.0 
38.5 
38.8 
39-° 
39-3 
39-4 
39-3 
29.6 
39-5 
39-6 
39 -i 
38.9 
u 8 
it 
III 
38.0c. 
37-8 
38.2 
38.3 
38.6 
38.9 
39-3 
39-2 
39-5 
40.0 
39- 6 
39-2 
38.8 
u 1 
u 
IV 
37-7C. 
37-5 
36.9 
38.O 
38.5 
39- 1 
39-3 
4°*3 
40.4 
39-8 
39-7 
39-5 
39-1 
u 
u 
V 
38.1c. 
38.3 
38.4 
38.O 
38.2 
O 
00 
CO 
37-5 
37-9 
38.x 
38.2 
38.4 
38.1 
38.5 
Free fri 
Gland*. 
iC 
VI 
37-5C. 
37-4 
37-8 
37-6 
37-5 
37-5 
37-4 
37-5 
37-3 
37-5 
37-7 
37-8 
37-8 
41 
