678 
R. MIDDLETON. 
On the 13th of July the two probably glanderous animals, Nos. 
I and II of the table, were given the third injection upon the 
left shoulder; this was composed of 2ccm. of malleine and 3ccm. 
of a I per cent, carbolic water. 
The table below traces its effect: 
THIRD INJECTION JULY 13, 1892, 6 O’CLOCK A. M., 2 GRAMMES 
MALLEINE. 
6 A.M. 
8 A.M 
10 A.M 
12 M. 
2 P.M. 
4 P.M. 
6 P.M. 
8 P.M. 
I. 
Pulse. 
49 
40 
40 
38 
40 
42 
42 
44 
Five year old 
Res >. 
14 
14 
12 
16 
16 
14 
12 
12 
black mare. 
Temp. 
38.0 
38.1 
38.2 
37.9 
38.0 
38.3 
38.3 
38.2 
II. 
Pulse. 
36 
36 
40 
38 
44 
52 
56 
56 
Ten year old 
Resp. 
16 
16 
18 
20 
24 
24 
22 
26 
chestnut geld. 
Temp. 
37.5 
37.8 
38.1 
38.6 
39.1 
39.4 
39.5 
39.3 
The general health of all of the horses, with the exception of 
No. II, was not essentially disturbed; only this animal manifested 
important advance of temperature, respiration and pulse, together 
with great immobility and anorexia. 
The local lesion consisted of an oedematous, hot and painful 
enlargement at the point of introduction; in the others this was 
of less importance. 
According to the tabulated results it appears that only No. 
II was suffering from glanders. The autopsy of all the horses 
was held on the 14th July (excepting No. IV, which was killed 
on July 9). In I, III, IV and V no lesion of a glanderous nature 
could be detected despite the most trying investigation. On the 
other hand, No. II displayed the most conclusive evidence in 
great abundance—in the right lung a superficial, lobular, pneu¬ 
monic herd, which upon section showed several small, yellowish 
tubercles with softening of the center. 
Summing the various series of experiments which have been 
