684 
J. A. REPLOG-LE. 
efforts of destruction are most often aimed, and to test the 
acid on a spore-producing germ, the bacillus subtilis was 
used. The virulence of the staphylococcus pyogenes aureus 
used was demonstrated by it producing an abscess under the 
skin of a rabbit inoculated with it; also by it causing the 
death of a rabbit inoculated in the vein of the ear. 
Experiments. — Series I. 
Throughout the first series of experiments the organisms 
were exposed to a two per cent, solution of the acid, and the 
experiments all made the same. 
Plate 1 .—September 8, i. cc. bouillon culture of the au¬ 
reus 3 days old ; 23.5 cc. of distilled sterilized water ; .5 cc. 
acid; control plate, too many to count; immediately, 48 ; 
five minutes, 5 ; ten minutes, o. Counted after 72 hours. 
Plate II.—September 12, bouillon culture of aureus 7 
days old ; control plate, too many to count; immedia’ely, too 
many to count; 5 minutes, 23 ; 10 minutes, o. Counted after 
72 hours. ' • 
Plate III.—September 14, bouillon culture 3 days old; 
control plate, too many to count; immediately, 97 ; 5 min¬ 
utes, 1 ; 10 minutes, o. Counted after 48 hours. 
Plate IV.—September 16, bouillon culture 24 hours old ; 
control plate, 68 ; immediately, 2 ; 5 minutes, o; 10 minutes, 
o. Counted after 48 hours. 
Plate V.—September 17, bouillon culture 48 hours old; 
control plate, 89; immediately, 1; 5 minutes, o ; 10 minutes,! 
o. Counted after 48 hours. 
Plate VI.—September 18, bouillon culture 72 hours old ; 
control plate, too many to count; immediately, 61 ; 5 min¬ 
utes, o ; 10 minutes, o. Counted after 72 hours. 
Plate VII.—September 21, bouillon culture 48 hours old ; 
control plates, 710; immediately, 2; 5 minutes, o; 10 min¬ 
utes, o. Counted after 72 hours. 
Plate VIII.—September 22, bouillon culture 4 days old; 
control plate, estimated at 8,640; immediately, 34 ; 5 min¬ 
utes, o ; 10 minutes, o. Counted after 72 Tours. 
Plate IX.—September 22, bouillon culture 48 hours old ; 
