686 
J. A> REPLOGLE. 
control plate, 239; immediately, o; 5 minutes, o; 10 min¬ 
utes, o. Counted after 72 hours. 
Plate IX.—October 3, bouillon culture 14 days old; con¬ 
trol plate, 198 ; immediately, o; 5 minutes, o; 10 minutes, o. 
Counted after 72 hours. 
Plate X.—October 4, bouillon culture 14 days old ; con¬ 
trol plate, 219; immediately, 3 ; 5 minutes, o; 10 minutes, o. 
Counted after 72 hours. 
Series III. 
Throughout this series the organisms were exposed to a 
one per cent, solution of the acid. This solution was pre¬ 
pared by adding to 95 cc. of distilled sterilized water, 1 cc. of 
the acid, and as soon as the acid was thoroughly dissolved 24 
cc. of the solution was measured into a test tube, and 1 cc. of 
the bouillon culture of aureus was added. 
Plate I.—October 5, bouillon culture 24 hours old; con¬ 
trol plate, 151 ; immediately, 92; 5 minutes, 10; 10 minutes, 
o. Counted after 48 hours. 
Plate II.—October 5, bouillon culture 24 hours old; 
control plate, 198; 5 minutes, 102; 10 minutes, 20; 15 min¬ 
utes, o. Counted after 48 hours. 
Plate III.—October 5, bouillon culture 48 hours old ; 
control plate, 648; immediately, 290; 5 minutes, 119; 10 
minutes, 7. Counted after 48 hours. 
Series IV. 
Organisms suspended in a water solution, and exposed to a 
ii per cent, solution of the acid. Prepared as follows : 5 cc. 
distilled sterilized water; 15 drops bouillon culture of aure¬ 
us; 4 cc. acid solution, 3:100. For the control tube, 10 cc. of 
distilled sterilized water, 15 drops of the same bouillon culture. 
Plate I.—October 8, aureus culture 3 days old. Control 
plate, estimated at 1,265; immediately, 112; 5 minutes, o; 
10 minutes, o. Counted after 48 hours. 
Plate II.—October 8, aureus culture 5 days old. Con¬ 
trol plate, estimated at 2,194 ; immediately, 185 ; 5 minutes, o ; 
10 minutes, o. Counted after 48 hours. 
Plate III.—October 10, made the same as plates I and II, 
using sterilized bouillon in the place of the water, as in I and 
mma ae tasm 
