DISINFECTING PROPERTIES OF CARBOLIC ACID. 
687 
II. Bouillon culture of aureus 5 days old. Control plate, 
estimated at 1,293 ; immediately, 81 ; 5 minutes, o; 10 minutes, 
o. Counted after 48 hours. 
Plate IV.—October 10, aureus culture 5 days old. Con¬ 
trol plate, estimated at 2,136 ; immediately o; 5 minutes, o ; 10 
minutes, o. 
Series V. 
The bacillus subtilis was used in this series to demonstrate 
the action of carbolic acid on spores. In the first three plates 
of this series, the organisms were exposed to a 1 \ per cent, so¬ 
lution of the acid. These plates were all made from the same 
bouillon culture of the organisms, 3 days old, which was filtered 
through glass wool to remove the enormous clumps which 
formed in the tJouillon, thus making the result more uniform. 
Plate I.—October 14. Control plate, estimated at 1170; 
immediately, estimated at 1,057; 5 minutes, estimated at 804; 
10 minutes, estimated at 284. Counted after 3 days. 
Plate II.—October 14, bouillon culture of subtilis 48 hours 
old. Control plate, estimated at 4,360 ; immediately estimated 
at 2,620; 5 minutes, estimated at 1,200; 10 minutes estimated 
at 630. Counted after 3 days. 
Plate III.—October 14, bouillon culture of subtilis 48 
hours old. Control plate, estimated at 930 ; immediately esti¬ 
mated at 568 ; 5 minutes, estimated at 420; 10 minutes, esti¬ 
mated at 210. Counted after 3 days. 
In the three following plates, the organisms were exposed 
to a 2 per cent, solution of the acid, the bouillon culture 6 
days old, filtered through glass wool. 
Plate IV.—October 17, bouillon culture of subtilis 48 
hours old. Control plate, estimated at 1,218; immediately 
estimated at 936 ; 5 minutes, estimated at 520; 10 minutes, es¬ 
timated at 118. Counted after 3 days. 
Plate V.—October 17, bouillon culture 4 days old. Con¬ 
trol plate, too many to count ; 5 minutes, estimated at 1,400; 
10 minutes, estimated at 1,100; 15 minutes, estimated at 296. 
Counted after 72 hours. 
Plate VI.—October 17, bouillon culture 4 days old. Con¬ 
trol plate, too many to count; 5 minutes, estimated at 1,680; 
