STUDY OF SPINAL CORD OF SPRING-HALT HORSE. 
691 
thinned and pared down, and the frog thoroughly cleaned, 
and kept so. The details of his whole treatment are unnec¬ 
essary. It will be sufficient to say that after two months’ care 
and treatment the animal improved greatly. Dr. Howard 
sa) s of this treatment, “that it caused a certain decrease of 
the excessive motion described, and we saw him on two or 
three occasions even start off without his customary intro¬ 
ducing ‘ ballet.’ ” The recovery was not complete, and the 
treatment does not seem to have given evidence that the ani¬ 
mal would ever have completely recovered, as it was de¬ 
stroyed. The spinal cord was taken out and sent to Dr. H. H. 
Donaldson, Assistant Professor of Neurology at Clark Uni¬ 
versity. He placed it in my hands, and under his direction 
it has been examined with a microscope. 
The specimen consisted of a section of the cord extending 
fiom the level corresponding to the eleventh dorsal vertebrae 
to the termination of the cord in the sacrum. The portion of 
the cord caudal of the origin of the fifth pair of lumbar nerves 
was so badly bruised in the process of removal from the ver¬ 
tebra that it was unfit lor microscopical examination, save the 
roots and ganglia of the first and second pairs of sacral nerves, 
which were held intact by the dura mater. In addition to 
this were the roots and ganglia of the fourth, fifth and sixth 
pairs of lumbar nerves. The whole specimen was hardened 
in a 2-5 per cent, solution of bichromate of potash plus one- 
sixth its volume of 95 per cent, alcohol. In this fluid it re¬ 
mained for two months, when sections were taken from six 
different levels of the cord, and also the roots and ganglia of 
the fourth, fifth and sixth pairs of lumbar, and of the first 
pairs of sacral nerves, and completely hardened in strong al¬ 
cohol. These specimens were then numbered to preserve the 
order, and sectioned. In staining, two purposes were kept 
in view: the first to show the fibres, and the second to show 
the cells. For fibre staining the best results were obtained 
with a double stain of palladium chloride and ammonia car¬ 
mine. Other fibre stains were nigrosin, acid fuchsin and car- 
minic acid. The most satisfactory cell stain was a double 
stain of Delafield’s hasmatoxylin, and Van Giesen’s picro- 
