Apr. 2* 1917 
Some Facts About Abortion Disease 
13 
the right front teat of the cow. The method of injection was through gravity, and 
the pressure used did not exceed that exerted by a column of fluid 12 inches high. 
Two guinea pigs were injected with samples of the suspension, and both later showed 
typical lesions of the kind caused in guinea pigs by abortion bacilli. 
April 8, 1916. Five days after the injection agglutination tests with blood serum 
from the cow were negative. 
April 17, 1916. Two weeks after the injection agglutination tests with blood serum 
from the cow were positive with dilutions of 1 to 400, which must be regarded as a 
very strong reaction. 
April 22, 1916. Material from the infected quarter of the cow's udder was injected 
into guinea pigs, which subsequently developed typical abortion-bacillus lesions. 
May 3, 1916. Material from each quarter of the cow's udder was injected separately 
into guinea pigs, all of which subsequently developed typical abortion-bacillus 
lesions, showing that the infection originally introduced into one quarter had spread 
to the other three quarters. On the same day material from the udder agglutinated 
suspensions of abortion bacilli in the following dilutions: 
Right front, or injected quarter. 1 to 6,400 
Left front quarter. 1 to 1,600 
Right hind quarter. 1 to 800 
Left hind quarter.. 1 to -1,600 
It is interesting to note how much higher the agglutinating value of 
material from the injected quarter is than from the other quarters. 
The material obtained from the cow's udder is not called “milk/' because 
the cow was practically dry; and it is questionable whether the material 
which can be stripped from a practically dry udder shortly before partu¬ 
rition can reasonably be looked upon as milk. 
May 9, 1916. The agglutinating value of material from the injected quarter of the 
udder was positive in a dilution of 1 to 12,800, and on May 15, 19, and 24, in a dilu¬ 
tion of 1 to 25,600. On these days the agglutinating value for suspensions of abor¬ 
tion bacilli of material from the other quarters of the udder remained constant for 
a dilution of 1 to 1,600, and that of the blood serum of the cow for a dilution of 1 to 400. 
May 26, 1916 (279 days after service by the bull). The cow produced an under¬ 
sized, weak calf, which, however, rapidly gained strength and is now a normal, 
healthy, vigorous animal. On the day of parturition the following agglutination 
tests were made: 
Colostrum, injected quarter of udder, positive, dilution 1 to 25,600. 
Colostrum, other three-quarters of udder, positive, dilution 1 to 1,600. 
Blood serum, cow, positive, dilution 1 to 400. 
Blood serum, calf, positive, dilution 1 to 400. 
When agglutination tests are made with blood serum, it is common 
for newly bom calves of infected cows to react in the same dilutions 
or quite as strongly as their mothers, but this power to react does not 
persist; it is a rapidly declining phenomenon, as is well shown by the 
following tests of the blood serum of the calf concerned in this record. 
On the day of its birth, as above recorded, the agglutination value 
of the calf's blood serum and that of its mother were identical; positive 
in dilutions of 1 to 400. Seven days later, June 2, the agglutination 
value of the calf's blood had declined to 1 to 200; on June 7 it had 
fallen to 1 to 100; on June 9 it was still at 1 to 100; but on July 10 
