May 7,1917 
Effects of Exercise and Sun on Normal Pigs 171 
2.—differential curve 
In five experiments an attempt was made to follow these changes in 
the differential count until the normal proportions returned. Spreads 
were made at intervals of a few hours and covering a period of 24 to 48 
hours. 
(a) Lymphocyte curve. —The lymphocytes were decreased, the 
height of the reaction occurring from two to seven hours after exercise, 
with an average of four hours. At this time they were decreased 29.43 
to 39.77 per cent, the average being 32.75 per cent. After the height 
of the reaction there was a gradual return to the normal proportions, 
which took place after 36 to 48 hours. 
(b) Polymorphonuclear curve. —The polymorphs were increased, 
the height of the reaction occurring from two to seven hours after exer¬ 
cise, and the average high point of the reaction was four hours after 
exercise. At this time they were increased 33.79 to 44.77 per cent, 
with an average increase of 39.88 per cent for the entire five animais. 
Following the height of the reaction the normal proportions gradually 
returned, and the time required varied between 36 and 48 hours. 
(c) Eosinophile curve. —The eosinophile curve varied from that 
found in the lymphocytes and polymorphs in that these cells showed a 
double curve. Shortly after the exercise (4.5 hours) the eosinopbiles 
dropped to zero, or nearly zero; following this there was a. gradual in¬ 
crease until these cells became much higher than the percentage nor¬ 
mally found. The height of this latter curve was reached about 24 
hours after exercise. Following this, the normal proportion gradually 
returned, and the count was again normal at 30 hours or longer. 
Table II details the changes in the eosinophile count in five experi¬ 
ments. 
Table II .—Changes in the percentages of eosinopbiles covering a period of 48 hours fol¬ 
lowing the muscular exercise of pigs 
Ani¬ 
mal 
No. 
Normal 
percent¬ 
age. 
Lowest 
percent 
age. 
Differ¬ 
ence. 
Hours 
after 
exercise 
when per¬ 
centage is 
lowest. 
Highest 
percent¬ 
age. 
• 
Hours 
after 
exercise 
when per¬ 
centage is 
highest. 
Hours after exercise when 
return to normal occurs. 
I. 
0. 56 
0.00 
Per cent. 
0. 56 
t 
4-5 
11.01 
24 
Not established. 
2. 
3-00 
.58 
3 - 42 
4*5 
9-68 
24 
Do. 
3 . 
I- 37 
.00 
i *37 
4*5 
11.88 
24 
Do. 
4 . 
13. 11 
•17 
12. 94 
7.0 
iS -<53 
24 
Do. 
5 . 
4. 82 
.00 
4.82 
2.0 
II. 02 
29 
About 30 hours. 
(d) MaST curve. —The behavior of the mast cells was very similar to 
that of the eosinophiles, and the curve was irregularly parallel with the 
eosinophile curve. 
82980°—17-2 
