85 
koch’s method with tuberculosis. 
plague blood, which had been repeatedly heated to 130°, 150° 
and 200° F., and a month later with an equal amount of viru¬ 
lent blood, which had been raised to 130° F. tor thirty min¬ 
utes, and the day following for three hours. This caused 
some loss of appetite and appearance of ill health, but no very 
appreciable fever. Thirteen days after the last operation 
this pig was placed in a small pen with a pig suffering from 
swine-plague, and at intervals of a month was twice inoculated 
with the virus of swine-plague, but all without evil conse¬ 
quence. 
“ Another pig was injected with a drachm of the mucus- 
covered fasces of a pig suffering from swine-plague, the infusion 
having first been filtered and heated for one-half hour to 130° 
F. until all movement of the contained bacteria had ceased. 
As in the other case, there was some evidence of ill health, but 
no material fever, and on the thirty-eighth day the subject 
was. placed in a small pen with a sick pig. Afterward, at in¬ 
tervals of a month, it was inoculated with (swine-plague) virus, 
but successfully resisted, and maintained good general health. 
“ A third pig was injected with a drachm of pork infusion, 
which had swarmed with bacteria resulting from an inocula¬ 
tion with infusion of putrid maize. Before inoculating upon 
the pig the pork infusion was heated to 140° F. for three 
hours in succession. There resulted some derangement of 
health, slight fever, and a local swelling in the seat of injec- 
tion. When this had subsided, on the fourteenth day, the pig 
was placed in a small pen in company with a diseased one. 
Nine days after she had a sharp attack of swine-plague, which 
lasted eighteen days and led to much loss of condition. Later, 
at intervals of a month, she was twice inoculated with active 
virus of swine-plague, but on each occasion without any fur¬ 
ther ill result. 
“ On the last occasion of the inoculation of these three 
pigs a fresh pig was inoculated with the same virulent matter, 
which caused considerable fever, with a temperature varying 
from 104° to 106 0 F., but from which the subject finally re¬ 
covered. 
“ Here, then, we have two pigs protected from the noxious 
